Bittilo Bon G, Cazzolato G, Avogaro P
J Lipid Res. 1981 Aug;22(6):998-1002.
HDL2 and HDL3 subclasses of human serum HDL were isolated by preparative ultracentrifugation and further analyzed by isotachophoraesis on polyacrylamide gel. The HDL2 divided into six subfractions and the HDL3 into ten subfractions differing in chemical composition and in apolipoproteinl content. The apoA-I/apoA-II ratios differed widely among the various subfractions. The subfractions with the highest apoA-I/apoA-II ratio appeared to have the greatest affinity for cholesterol. The preparative isotachophoresis, used for the first time in this type of investigation, has high resolving power and is reproducible and thus suitable for use in the study of the structure and metabolism of the lipoproteins.
人血清高密度脂蛋白（HDL）的HDL2和HDL3亚类通过制备性超速离心法分离，并在聚丙烯酰胺凝胶上通过等速电泳进一步分析。HDL2分为六个亚组分，HDL3分为十个亚组分，它们在化学组成和载脂蛋白含量上有所不同。不同亚组分之间的载脂蛋白A-I/载脂蛋白A-II比率差异很大。载脂蛋白A-I/载脂蛋白A-II比率最高的亚组分似乎对胆固醇具有最大的亲和力。首次用于此类研究的制备性等速电泳具有高分辨率、可重复性，因此适用于脂蛋白结构和代谢的研究。
