Adams N R
J Endocrinol. 1981 Sep;90(3):309-14. doi: 10.1677/joe.0.0900309.
Ovariectomized ewes treated with oestradiol benzoate (OB) had a reduced cervical mucus response to subsequent treatment with OB. This refractory effect was observed 48 h after a single dose of OB, but could be postponed until 72 h by a second dose of OB at 24 h. This postponement did not occur in animals which were recovering from previously induced refractoriness. Less mucus was recovered from refractory ewes, and it had an increased proportion of dry matter and normal Spinnbarkeit. Pretreatment of ewes with 10 mg progesterone for 10 days decreased the proportion of dry matter and increased the Spinnbarkeit of mucus collected after stimulation with OB. This change was different from that produced by increasing the dose of OB. Progesterone given to ewes after 10 days treatment with OB had a detectable effect on cervical mucus, but it did not specifically decrease refractoriness. The cervix provides a convenient site for studying oestrogen-induced refractoriness in the ewe, without possible confusion by an effects of progesterone.
用苯甲酸雌二醇（OB）处理的去卵巢母羊，对随后用OB进行的处理，其宫颈黏液反应降低。在单次注射OB后48小时观察到这种不应性效应，但在24小时给予第二剂OB可将其推迟至72小时。这种推迟在从先前诱导的不应性中恢复的动物中未发生。从不应性母羊中回收的黏液较少，且干物质比例增加，拉丝度正常。用10毫克孕酮对母羊进行10天预处理，可降低干物质比例，并增加用OB刺激后收集的黏液的拉丝度。这种变化与增加OB剂量所产生的变化不同。在用OB处理10天后给母羊注射孕酮，对宫颈黏液有可检测到的影响，但它并没有特异性地降低不应性。子宫颈为研究母羊雌激素诱导的不应性提供了一个便利的部位，不会因孕酮的影响而产生混淆。
