Gerber-Taras E, Park B K, Ohnhaus E E
J Clin Chem Clin Biochem. 1981 Aug;19(8):525-7. doi: 10.1515/cclm.1981.19.8.525.
In the present study two recently developed methods, a high performance liquid chromatography (HPLC) and a radioimmunoassay (RIA) for the estimation of 6 beta-hydroxycortisol in urine were compared. Both methods showed a very reliable intraassay variation of about 5.2 or 7.2% respectively. Using the baseline values of excretion during a 24-hour collecting period in human urine, and after induction of the liver microsomal enzyme system by different substances, both methods gave identical values. The HPLC-method still needs an extraction step at the beginning of the analytical procedure, while a direct measurement in the urine can be performed with the RIA. Furthermore, the RIA is not time consuming and is easy to perform. As the antiserum is highly specific there is no cross-reaction with other cortisol metabolites.
在本研究中，对两种最近开发的用于测定尿中6β-羟基皮质醇的方法进行了比较，即高效液相色谱法（HPLC）和放射免疫分析法（RIA）。两种方法的批内变异分别非常可靠，约为5.2%或7.2%。利用人尿24小时收集期内的排泄基线值，以及在不同物质诱导肝微粒体酶系统后，两种方法得出的结果相同。HPLC法在分析过程开始时仍需要一个提取步骤，而RIA法可直接对尿液进行测量。此外，RIA法不耗时且易于操作。由于抗血清具有高度特异性，与其他皮质醇代谢产物无交叉反应。
