Hydén H, Lange P W
J Neurobiol. 1981 May;12(3):201-10. doi: 10.1002/neu.480120302.
Since antiserum raised against the S 100 protein has an impairing effect on acquisition in behavioral tests, when interacting with S 100 on hippocampal cells, the effect of S 100 antiserum was studied in rats on the S 100 content of the hippocampus and thalamus, as well as on behavior. The operant reversal of handedness test and a light discrimination test were used. S 100 antiserum, 2 X 30 mu l, was injected intraventricularly before and during the sessions of two different learning tests. The S 100 protein was determined by quantitative immunoelectrophoresis. In the antiserum-injected animals, the levels of S 100 protein was increased by up to 30%, the incorporation values of 3H-valine increased in proteins of high molecular weight. Further acquisition was inhibited compared to controls, in which antiserum absorbed with pure S 100 protein was injected intraventricularly. The stimulation of S 100 synthesis, probably by the glia, may have occurred by a negative feedback effect, as has been observed in thymocytes.
由于针对S100蛋白产生的抗血清在行为测试中对习得有损害作用，当与海马细胞上的S100相互作用时，在大鼠中研究了S100抗血清对海马和丘脑S100含量以及行为的影响。使用了利手操作反转测试和光辨别测试。在两种不同学习测试的过程之前和期间，向脑室内注射2×30μl的S100抗血清。通过定量免疫电泳测定S100蛋白。在注射抗血清的动物中，S100蛋白水平升高高达30%，3H-缬氨酸在高分子量蛋白质中的掺入值增加。与向脑室内注射用纯S100蛋白吸收的抗血清的对照组相比，进一步的习得受到抑制。如在胸腺细胞中所观察到的，可能由神经胶质细胞刺激S100合成，这可能是通过负反馈效应发生的。
