McQuade P S, Juorio A V, Boulton A A
J Neurochem. 1981 Sep;37(3):735-9. doi: 10.1111/j.1471-4159.1982.tb12549.x.
The mouse brain contains 12.5 and 4.1 ng/g of p- and m-hydroxyphenylacetic acids, respectively. The hydroxyphenylacetic acids were isolated by chromatography on DEAE-Sephadex A-25 and quantitated as their pentafluoropropionyl and hexafluoropropanol esters by use of a gas chromatograph equipped with an electron-capture detector. The highest concentrations of p- or m-hydroxyphenylacetic acids were observed in the caudate nuclei (27.9 and 8.7 ng/g, respectively) and olfactory tubercles (20.2 and 5.3 ng/g, respectively). The identities of the p- and m-hydroxyphenylacetic acids were further confirmed as a consequence of the reductions observed following monoamine oxidase inhibition or the increases observed in the appropriate acid following the parenteral administration of p- or m-tyramine.
小鼠大脑中对羟基苯乙酸和间羟基苯乙酸的含量分别为12.5纳克/克和4.1纳克/克。通过在DEAE-葡聚糖A-25上进行色谱分离来分离羟基苯乙酸，并使用配备电子捕获检测器的气相色谱仪将其定量为五氟丙酰酯和六氟丙醇酯。在尾状核（分别为27.9纳克/克和8.7纳克/克）和嗅结节（分别为20.2纳克/克和5.3纳克/克）中观察到对羟基苯乙酸或间羟基苯乙酸的最高浓度。由于单胺氧化酶抑制后观察到含量降低，或在腹腔注射对酪胺或间酪胺后相应酸的含量增加，进一步证实了对羟基苯乙酸和间羟基苯乙酸的特性。
