Davis B A, Durden D A, Pun-Li P, Boulton A A
J Chromatogr. 1977 Nov 11;142:517-22. doi: 10.1016/s0021-9673(01)92064-3.
m- and p-Hydroxyphenylacetic acids have been identified and quantitated in rat brain by gas chromatography of their pentafluoropropionyl hexafluoroisopropyl diesters. The procedure differs from those employed in earlier studies on the catecholic acids in that a support-coated open-tubular column was used, a preliminary purification of the acids by thin-layer chromatography was carried out, and excess derivatizing reagent was removed by extraction with a phosphate buffer.
已通过气相色谱法，利用间羟基苯乙酸和对羟基苯乙酸的五氟丙酰基六氟异丙基二酯对大鼠脑中的这两种物质进行了鉴定和定量。该方法与早期对儿茶酚酸的研究方法不同，具体在于使用了载体涂渍空心柱，通过薄层色谱法对酸进行了初步纯化，并用磷酸盐缓冲液萃取去除了过量的衍生试剂。
