Schloerb P R, Palaskas C L, Mintun M A
J Trauma. 1981 Sep;21(9):757-61. doi: 10.1097/00005373-198109000-00002.
Using computer analysis of the early plasma arterial disappearance curve of tritiated water (HTO), we sought the fewest points and earliest times needed to predict the final volume of dilution, total body water (TBW). In ten anesthetized adult female dogs weighing 19.1 +/- 0.5 kg, with bilateral ureteral ligation, 500 muC HTO were given IV. Arterial blood samples were taken until equilibrium (3 hours), when the approximate equivalent of extracellular fluid (ECF), 4,000 ml of lactated Ringer's solution, was given IV within 1 hour. The next day, in the second phase of the study, 1,000 muC of HTO were given IV and arterial blood samples were taken at intervals up to equilibrium (5 hours). TBW at 3 hours after the first HTO infusion was 63.3 +/- 1.2% body weight. Using a curve-fitting Fortran program (CFIT), the arterial plasma HTO concentrations were fitted to one or two exponentials. Although initial TBW could be predicted from arterial plasma concentrations of HTO during 20 minutes after injection in normally hydrated dogs, values during 60 minutes were required for accurate prediction of TBW after infusion of 4 L of fluid. TBW in normal and fluid-loaded animals was predicted within 2.3 +/- 0.6% of the final HTO equilibrium (r = 0.987).
通过对氚标记水（HTO）早期血浆动脉消失曲线进行计算机分析，我们探寻预测稀释终体积即总体水（TBW）所需的最少点数和最早时间。对10只体重为19.1±0.5千克的成年雌性麻醉犬进行双侧输尿管结扎，静脉注射500μC HTO。采集动脉血样本直至达到平衡状态（3小时），此时在1小时内静脉注射约相当于细胞外液（ECF）的4000毫升乳酸林格氏液。第二天，在研究的第二阶段，静脉注射1000μC HTO，并每隔一定时间采集动脉血样本直至达到平衡状态（5小时）。首次注射HTO后3小时的TBW为体重的63.3±1.2%。使用曲线拟合Fortran程序（CFIT），将动脉血浆HTO浓度拟合为一个或两个指数函数。尽管在正常水合状态的犬中，注射后20分钟内的动脉血浆HTO浓度可预测初始TBW，但输注4升液体后，准确预测TBW需要60分钟内的值。正常动物和液体负荷动物的TBW预测值与HTO最终平衡值的偏差在2.3±0.6%以内（r = 0.987）。
