Teramoto K, Horiguchi S
Sangyo Igaku. 1980 Sep;22(5):368-71. doi: 10.1539/joh1959.22.368.
For monitoring solvents exposure, it is useful to determine not only metabolites of the solvents in urine but also the solvents themselves in blood and tissues. In a series of studies on the industrial styrene poisoning, we have been determining styrene in blood and other tissues as occasion calls. Our examination of the method is presented in this report. The outline on the method is as follows: Aliquots of 0.5g of tissues being added 5 ml of n-hexane are homogenized by a high-speed homogenizer (Polytoron) for 10 to 30 seconds and the filtrates containing extracts are analyzed for styrene by gas chromatography. The linearity was ascertained from the calibration curve obtained by adding the known quantities of styrene (4, 10, 20, 40, 100 ppm) to the blood, liver and adipose tissues of rats. Rates of recoveries of styrene from the above tissues were 92 to 101 per cent. Reproducibility of this method was examined by repeating determinations of styrene in the blood, liver and adipose tissues of rats exposed to 500 ppm styrene for 4 hours, the coefficients of variation being 2.8 to 14.0 per cent. There was an approximately linear relationship between the styrene concentration (0 to 1,000 ppm) of the exposed air and those in the blood of exposed rats. We conclude that our simple and rapid method is applicable to determination of solvents other than styrene in organs and tissues by combining suitable solvents for extraction and packings of gas chromatography.
为监测溶剂暴露情况，不仅测定尿液中溶剂的代谢产物，同时测定血液和组织中的溶剂本身是很有用的。在一系列关于工业苯乙烯中毒的研究中，我们根据需要测定血液和其他组织中的苯乙烯。本报告介绍了我们对该方法的检验。该方法概述如下：将0.5g组织的等分试样加入5ml正己烷，用高速匀浆器（Polytoron）匀浆10至30秒，然后用气相色谱法分析含有提取物的滤液中的苯乙烯。通过向大鼠的血液、肝脏和脂肪组织中添加已知量的苯乙烯（4、10、20、40、100ppm）得到校准曲线，从而确定线性关系。苯乙烯从上述组织中的回收率为92%至101%。通过重复测定暴露于500ppm苯乙烯4小时的大鼠血液、肝脏和脂肪组织中的苯乙烯来检验该方法的重现性，变异系数为2.8%至14.0%。暴露空气中苯乙烯浓度（0至1000ppm）与暴露大鼠血液中的苯乙烯浓度之间存在近似线性关系。我们得出结论，通过结合适合的提取溶剂和气相色谱填充剂，我们的简单快速方法适用于测定器官和组织中除苯乙烯以外的溶剂。
