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1
Inhibitory effects of 3'deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate on DNA-dependent RNA polymerases I and II purified from Dictyostelium discoideum cells.3'-脱氧胞苷5'-三磷酸和3'-脱氧尿苷5'-三磷酸对从盘基网柄菌细胞中纯化的依赖DNA的RNA聚合酶I和II的抑制作用。
Nucleic Acids Res. 1981 Jul 10;9(13):3129-38. doi: 10.1093/nar/9.13.3129.
2
Inhibitory effects of 3'-modified UTP analogues on DNA-dependent RNA polymerase I and II purified from cherry salmon (Onchorhynchus masou) liver.3'-修饰的UTP类似物对从樱花鲑（Onchorhynchus masou）肝脏中纯化的DNA依赖性RNA聚合酶I和II的抑制作用。
Nucleic Acids Symp Ser. 1981(10):143-6.
3
Template specificity of DNA-dependent RNA polymerase I and II for synthetic polynucleotides during development of the cellular slime mold Dictyostelium discoideum.在细胞黏菌盘基网柄菌发育过程中，DNA依赖的RNA聚合酶I和II对合成多核苷酸的模板特异性。
J Biochem. 1980 May;87(5):1501-9. doi: 10.1093/oxfordjournals.jbchem.a132891.
4
Inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate on DNA-dependent RNA polymerase I and II from cherry salmon (Oncorhynchus masou).9-β-D-木糖呋喃糖基腺嘌呤5'-三磷酸对樱鳟（Oncorhynchus masou）DNA依赖性RNA聚合酶I和II的抑制作用
J Biochem. 1985 May;97(5):1385-9. doi: 10.1093/oxfordjournals.jbchem.a135192.
5
Differential inhibitory effects of 5-substituted 1-beta-D-xylofuranosyluracil 5'-triphosphates and related nucleotides on DNA-dependent RNA polymerases I and II from the cherry salmon (Oncorhynchus masou).5-取代的1-β-D-呋喃木糖基尿嘧啶5'-三磷酸及相关核苷酸对樱鳟（Oncorhynchus masou）DNA依赖性RNA聚合酶I和II的差异抑制作用
J Biochem. 1985 Aug;98(2):417-25. doi: 10.1093/oxfordjournals.jbchem.a135296.
6
Characterization of a novel dUTP-dependent activity of CTP synthetase from Saccharomyces cerevisiae.酿酒酵母CTP合成酶一种新型dUTP依赖性活性的表征
Biochemistry. 1999 Dec 14;38(50):16671-7. doi: 10.1021/bi9920127.
7
Purification and subunit structure of RNA polymerases I and II from Dictyostelium discoideum vegetative cells.盘基网柄菌营养细胞中RNA聚合酶I和II的纯化及亚基结构
Mol Cell Biochem. 1985 Feb;66(1):21-9. doi: 10.1007/BF00231819.
8
The mutations induced by oxidatively damaged nucleotides, 5-formyl-dUTP and 5-hydroxy-dCTP,in Escherichia coli.氧化损伤核苷酸5-甲酰基-dUTP和5-羟基-dCTP在大肠杆菌中诱导的突变。
Nucleic Acids Res. 1998 Oct 15;26(20):4582-7. doi: 10.1093/nar/26.20.4582.
9
[Inhibitory effect of 3'-azido-3'-azido-3'-deoxy- ribavirin-5'-triphosphate on RNA synthesis catalyzed by RNA polymerase from influenza A virus and by cellular DNA-dependent RNA polymerase II].[3'-叠氮-3'-叠氮-3'-脱氧-利巴韦林-5'-三磷酸对甲型流感病毒RNA聚合酶及细胞DNA依赖性RNA聚合酶II催化的RNA合成的抑制作用]
Vopr Med Khim. 1989 Jan-Feb;35(1):104-6.
10
Utilizations of various uridine 5'-triphosphate analogues by DNA-dependent RNA polymerases I and II purified from liver nuclei of the cherry salmon (Oncorhynchus masou).从樱桃三文鱼（Oncorhynchus masou）肝细胞核中纯化的DNA依赖性RNA聚合酶I和II对各种尿苷5'-三磷酸类似物的利用情况。
J Biochem. 1984 Nov;96(5):1501-9. doi: 10.1093/oxfordjournals.jbchem.a134979.

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Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata.从嗜热放线菌弯曲热单孢菌中分离依赖DNA的RNA聚合酶。
Folia Microbiol (Praha). 1994;39(1):7-12. doi: 10.1007/BF02814521.
2
Purification of DNA-dependent RNA polymerase from Streptomyces granaticolor.从石榴色链霉菌中纯化依赖DNA的RNA聚合酶。
Folia Microbiol (Praha). 1983;28(5):435-8. doi: 10.1007/BF02879496.
3
Complex RNA chain elongation kinetics by wheat germ RNA polymerase II.小麦胚芽RNA聚合酶II的复杂RNA链延伸动力学
Nucleic Acids Res. 1984 Apr 11;12(7):3303-19. doi: 10.1093/nar/12.7.3303.
4
Some properties of DNA-dependent RNA polymerase from Streptomyces granaticolor.石榴链霉菌依赖DNA的RNA聚合酶的一些特性
Folia Microbiol (Praha). 1985;30(6):517-20. doi: 10.1007/BF02927616.
5
Effect of low nucleotide concentrations on abortive elongation catalysed by wheat-germ RNA polymerase II.低核苷酸浓度对小麦胚芽RNA聚合酶II催化的流产性延伸的影响。
Biochem J. 1987 May 15;244(1):151-7. doi: 10.1042/bj2440151.
6
Effect of granaticin on the activity of DNA-directed RNA polymerase from Streptomyces granaticolor.石榴菌素对石榴链霉菌DNA指导的RNA聚合酶活性的影响。
Folia Microbiol (Praha). 1989;34(6):536-8. doi: 10.1007/BF02814465.
7
Isolation of DNA-dependent RNA polymerase from Streptomyces granaticolor and its binding to phage phi 29 DNA.从石榴链霉菌中分离DNA依赖性RNA聚合酶及其与噬菌体φ29 DNA的结合
Folia Microbiol (Praha). 1991;36(2):120-6. doi: 10.1007/BF02814489.
8
Effect of protein phosphorylation on the activity of RNA polymerase in streptomycetes.蛋白质磷酸化对链霉菌中RNA聚合酶活性的影响。
Folia Microbiol (Praha). 1992;37(1):53-9. doi: 10.1007/BF02814581.

本文引用的文献

1
Specific inhibition of chromatin-associated poly(A) synthesis in vitro by cordycepin 5'-triphosphate.虫草素5'-三磷酸对体外染色质相关多聚腺苷酸合成的特异性抑制作用。
Nature. 1977 May 12;267(5607):178-80. doi: 10.1038/267178a0.
2
CONVERSION OF MONO- AND OLIGODEOXYRIBONUCLEOTIDES TO 5-TRIPHOSPHATES.单脱氧核糖核苷酸和寡脱氧核糖核苷酸向5-三磷酸酯的转化。
J Am Chem Soc. 1965 Apr 20;87:1785-8. doi: 10.1021/ja01086a031.
3
THE INHIBITION OF PHOSPHORIBOSYL-PYROPHOSPHATE AMIDOTRANSFERASE ACTIVITY BY CORDYCEPIN MONOPHOSPHATE.单磷酸虫草素对磷酸核糖焦磷酸酰胺转移酶活性的抑制作用
Biochim Biophys Acta. 1964 Sep 18;89:465-72. doi: 10.1016/0926-6569(64)90072-0.
4
Synthetic nucleosides and nucleotides. XVI. Synthesis and biological evaluations of a series of 1-beta-D-arabinofuranosylcytosine 5'-alkyl or arylphosphates.合成核苷与核苷酸。十六。一系列1-β-D-阿拉伯呋喃糖基胞嘧啶5'-烷基或芳基磷酸酯的合成与生物学评价。
Chem Pharm Bull (Tokyo). 1980 Oct;28(10):2915-23. doi: 10.1248/cpb.28.2915.
5
Template specificity of DNA-dependent RNA polymerase I and II for synthetic polynucleotides during development of the cellular slime mold Dictyostelium discoideum.在细胞黏菌盘基网柄菌发育过程中，DNA依赖的RNA聚合酶I和II对合成多核苷酸的模板特异性。
J Biochem. 1980 May;87(5):1501-9. doi: 10.1093/oxfordjournals.jbchem.a132891.
6
A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose.从微球菌（溶壁微球菌）中分离得到的一种脱氧核糖核酸聚合酶，该酶是在脱氧核糖核酸 - 纤维素上分离得到的。
J Biol Chem. 1968 Dec 10;243(23):6222-33.
7
Specific inhibition of nuclear RNA polymerase II by alpha-amanitin.α-鹅膏蕈碱对核RNA聚合酶II的特异性抑制作用。
Science. 1970 Oct 23;170(3956):447-9. doi: 10.1126/science.170.3956.447.
8
Messenger RNA formation: resistance to inhibition by 3'-deoxycytidine.信使核糖核酸的形成：对3'-脱氧胞苷抑制作用的抗性。
Biochim Biophys Acta. 1972 Aug 16;277(1):129-33. doi: 10.1016/0005-2787(72)90359-0.
9
Characteristics of the polyadenylic acid segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cells.与小鼠肉瘤180腹水细胞中信使核糖核酸相关的聚腺苷酸片段的特征
Biochemistry. 1972 Feb 29;11(5):792-8. doi: 10.1021/bi00755a018.
10
Evidence for two alpha-amanitin-resistant RNA polymerases in vegetative amoebae of Dictyostelium discoideum.盘基网柄菌营养期变形虫中存在两种抗α-鹅膏蕈碱RNA聚合酶的证据。
Biochem Biophys Res Commun. 1976 Jan 12;68(1):183-9. doi: 10.1016/0006-291x(76)90027-9.

3'-脱氧胞苷5'-三磷酸和3'-脱氧尿苷5'-三磷酸对从盘基网柄菌细胞中纯化的依赖DNA的RNA聚合酶I和II的抑制作用。

Inhibitory effects of 3'deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate on DNA-dependent RNA polymerases I and II purified from Dictyostelium discoideum cells.

作者信息

Saneyoshi M, Tohyama J, Nakayama C, Takiya S, Iwabuchi M

出版信息

Nucleic Acids Res. 1981 Jul 10;9(13):3129-38. doi: 10.1093/nar/9.13.3129.

DOI:10.1093/nar/9.13.3129
PMID:7279664
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327336/
Abstract

3'-Deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate were synthesized starting from cordycepin in good yield. The inhibitory effects of these nucleotides were examined in comparison with that of cordycepin 5'-triphosphate (3'-dATP) using purified DNA-dependent RNA polymerases I and II from Dictyostelium discoideum cells. Both nucleotide analogues strongly and competitively inhibited the incorporations of CTP and UTP into RNA by the RNA polymerases. The Km and Ki values for CTP and 3'-dCTP were 6.3 micro M and 3.0 micro M, respectively, and those for UTP and 3'-dUTP were 6.3 micro M and 2.0 micro M, respectively. These two analogues will be useful in studies at the molecular level on the relationship of template and substrate in RNA synthesis with chromatin, isolated nuclei or permeable cells, because they do not have any effect on poly (rA) synthesis.

摘要

以虫草素为原料，高产率合成了3'-脱氧胞苷5'-三磷酸和3'-脱氧尿苷5'-三磷酸。使用从盘基网柄菌细胞中纯化的依赖DNA的RNA聚合酶I和II，将这些核苷酸的抑制作用与虫草素5'-三磷酸（3'-dATP）的抑制作用进行了比较。这两种核苷酸类似物均强烈且竞争性地抑制RNA聚合酶将CTP和UTP掺入RNA中。CTP和3'-dCTP的Km和Ki值分别为6.3微摩尔和3.0微摩尔，UTP和3'-dUTP的Km和Ki值分别为6.3微摩尔和2.0微摩尔。这两种类似物在研究RNA与染色质、分离的细胞核或可渗透细胞合成过程中模板与底物之间的分子水平关系时将很有用，因为它们对聚（rA）合成没有任何影响。