Rondeau M, Rouleau M
Rev Can Biol. 1981 Jun;40(2):173-80.
Using semen from bull, boar and stallion as well as different spectrophotometers, we established the calibration curves relating the optical density of a sperm sample to the sperm count obtained on the hemacytometer. The results show that, for a given spectrophotometer, the calibration curve is not characteristic of the animal species we studied. The differences in size of the spermatozoa are probably too small to account for the anticipated specificity of the calibration curve. Furthermore, the fact that different dilution rates must be used, because of the vastly different concentrations of spermatozoa which is characteristic of those species, has no effect on the calibration curves since the dilution rate is shown to be artefactual. On the other hand, for a given semen, the calibration curve varies depending upon the spectrophotometry used. However, if two instruments have the same characteristic in terms of spectral bandwidth, the calibration curves are not statistically different.
我们使用公牛、公猪和种马的精液以及不同的分光光度计，建立了将精子样本的光密度与血细胞计数器上获得的精子计数相关联的校准曲线。结果表明，对于给定的分光光度计，校准曲线并非我们所研究动物物种的特征曲线。精子大小的差异可能太小，无法解释校准曲线预期的特异性。此外，由于这些物种精子浓度差异极大，必须使用不同的稀释率，但这对校准曲线并无影响，因为稀释率被证明是人为因素。另一方面，对于给定的精液，校准曲线会因所使用的分光光度法而异。然而，如果两台仪器在光谱带宽方面具有相同特性，则校准曲线在统计学上并无差异。
