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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Oron U, Kinamon S, Bdolah A

Biochem J. 1978 Sep 15;174(3):733-9. doi: 10.1042/bj1740733.

Venom of Vipera palastinae was subjected to isoelectrofocusing on polyacrylamide gel. The protein separation profiles were similar for different venom samples; more than 25 protein bands with a wide range of pI values could be demonstrated by this technique. 2. Labelled venom was obtained 8h after an intracardial injection of [3H]leucine. The relative radioactivities of four out of 12 main protein bands were significantly different in the venom synthesized during the 2nd day of the venom regeneration cycle as compared with the venom of the 4th day. The comparison was made in venom samples obtained from the two glands of the same snake at two different secretory stages. 3. It is concluded that the asynchronous synthesis of exportable proteins after the initiation of a new venom regeneration cycle is responsible for the non-parallel secretion of some venom proteins by the venom gland of Vipera palaestinae during the first few days after milking.

对巴勒斯坦蝰蛇的毒液进行聚丙烯酰胺凝胶等电聚焦。不同毒液样本的蛋白质分离图谱相似；通过该技术可显示出25条以上具有广泛pI值的蛋白带。2. 在心脏内注射[3H]亮氨酸8小时后获得标记毒液。与第4天的毒液相比，在毒液再生周期第2天合成的毒液中，12条主要蛋白带中的4条的相对放射性存在显著差异。该比较是在同一蛇的两个腺体在两个不同分泌阶段获得的毒液样本中进行的。3. 得出的结论是，新的毒液再生周期开始后可输出蛋白质的异步合成，是导致巴勒斯坦蝰蛇毒腺在采毒后的头几天内一些毒液蛋白非平行分泌的原因。

Oron U, Kinamon S, Bdolah A

Biochem J. 1978 Sep 15;174(3):733-9. doi: 10.1042/bj1740733.

Venom of Vipera palastinae was subjected to isoelectrofocusing on polyacrylamide gel. The protein separation profiles were similar for different venom samples; more than 25 protein bands with a wide range of pI values could be demonstrated by this technique. 2. Labelled venom was obtained 8h after an intracardial injection of [3H]leucine. The relative radioactivities of four out of 12 main protein bands were significantly different in the venom synthesized during the 2nd day of the venom regeneration cycle as compared with the venom of the 4th day. The comparison was made in venom samples obtained from the two glands of the same snake at two different secretory stages. 3. It is concluded that the asynchronous synthesis of exportable proteins after the initiation of a new venom regeneration cycle is responsible for the non-parallel secretion of some venom proteins by the venom gland of Vipera palaestinae during the first few days after milking.

对巴勒斯坦蝰蛇的毒液进行聚丙烯酰胺凝胶等电聚焦。不同毒液样本的蛋白质分离图谱相似；通过该技术可显示出25条以上具有广泛pI值的蛋白带。2. 在心脏内注射[3H]亮氨酸8小时后获得标记毒液。与第4天的毒液相比，在毒液再生周期第2天合成的毒液中，12条主要蛋白带中的4条的相对放射性存在显著差异。该比较是在同一蛇的两个腺体在两个不同分泌阶段获得的毒液样本中进行的。3. 得出的结论是，新的毒液再生周期开始后可输出蛋白质的异步合成，是导致巴勒斯坦蝰蛇毒腺在采毒后的头几天内一些毒液蛋白非平行分泌的原因。