Walker J R
Br J Cancer. 1978 Oct;38(4):513-20. doi: 10.1038/bjc.1978.238.
A malignant cell line derived from the s.c. inoculation of Adenovirus 12 into a CBA mouse has been isolated in vitro, cloned, and within 10 passages the clones have been investigated for their karyotype, morphology, growth rate, saturation density and response to plant lectin in vitro, and their tumorigenicity and growth rate in vivo. The cell lines rapidly acquired a highly heterogeneous karyotype, but remained homogeneous with respect to more complex physiological parameters. Examination of the cellular characteristics has indicated that the rate of growth of the cell lines in vivo, but not their tumorigenicity, may be related to their in vitro potentials. The clones responded differently to the cytotoxic effects of concanavalin A, but there was no correlation between the effect of the lectin and the malignant potential of the cells.
通过将腺病毒12接种到CBA小鼠皮下而获得的恶性细胞系已在体外分离、克隆，并在10代内对克隆进行了核型、形态、生长速率、饱和密度以及体外对植物凝集素的反应，及其在体内的致瘤性和生长速率的研究。这些细胞系迅速获得了高度异质性的核型，但在更复杂的生理参数方面仍保持同质性。对细胞特征的检查表明，细胞系在体内的生长速率而非其致瘤性可能与其体外潜能有关。这些克隆对伴刀豆球蛋白A的细胞毒性作用反应不同，但凝集素的作用与细胞的恶性潜能之间没有相关性。
