Vishenvskaia Z I, Sukhomudrenko A G, Lobyshev V I
Biofizika. 1981 Jul-Aug;26(4):751-3.
Melting temperature of myosin Tm against the concentration of D2O in the system was studied. The D2O/Tm relationship is analogous to that between the kinetics isotope effect and the volumic fraction of D2O in the system. This similarity as well as the difference between the observed kinetic and thermodynamic isotopic effects and the theoretical ones make us suggest that the conformational changes in myosin during ATP hydrolysis are rather substantial.
研究了肌球蛋白的解链温度（Tm）与体系中重水（D2O）浓度的关系。D2O/Tm关系类似于体系中动力学同位素效应与D2O体积分数之间的关系。观察到的动力学和热力学同位素效应与理论效应之间的这种相似性以及差异，使我们认为ATP水解过程中肌球蛋白的构象变化相当显著。
