Gedamu L, Chaconas G, van de Sande J H, Dixon G H
Biosci Rep. 1981 Jan;1(1):61-70. doi: 10.1007/BF01115150.
The structures of the 5' termini of the protamine mRNAs (PmRNAs) have been investigated by inhibiting their translation in wheat-germ extracts in the presence of 7-methyl guanosine 5'-phosphate (m7-GMP), an analogue of 'cap' structure in mRNAs. Second, the cap structures on PmRNAs were examined by labelling the RNA at the 5' end with T4 polynucleotide kinase and [gamma-32P]ATP before and after removal of these structures with tobacco acid pyrophosphatase and alkaline phosphatase. The results indicate that cap structures of the PmRNAs are heterogeneous. It appears that the mRNAs coding for protamine components CI and CIII have at least a cap 1 structure while the mRNAs coding for CII do not appear to be capped or methylated.
通过在7-甲基鸟苷5'-磷酸（m7-GMP，一种mRNA中“帽”结构的类似物）存在的情况下抑制其在小麦胚芽提取物中的翻译，对鱼精蛋白mRNA（PmRNAs）5'末端的结构进行了研究。其次，在用烟草酸焦磷酸酶和碱性磷酸酶去除这些结构之前和之后，通过用T4多核苷酸激酶和[γ-32P]ATP在5'末端标记RNA来检查PmRNAs上的帽结构。结果表明，PmRNAs的帽结构是异质的。编码鱼精蛋白组分CI和CIII的mRNA似乎至少具有帽1结构，而编码CII的mRNA似乎没有被加帽或甲基化。
