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通过聚焦激光束扫描对含细菌视紫红质的脂质双层的光电活性进行研究。

Investigation by focused laser beam scanning of the photoelectric activity of bacteriorhodopsin-containing lipid bilayers.

作者信息

Dancsházy Z, Ormos P, Drachev L A, Skulachev V P

出版信息

Biophys J. 1978 Nov;24(2):423-8. doi: 10.1016/S0006-3495(78)85392-2.

Abstract

The photoelectric activity of different parts of lipid bilayer containing bacteriorhodopsin was investigated by moving a small actinic light spot across the Plateau-Gibbs border and the bimolecular part of this reconstituted model membrane. The results give direct evidence that bacteriorhodopsin incorporated into the bimolecular region of the lipid membrane is responsible for the photoelectric activity of this system. A technique for scanning the photoelectric activity of a modified bimolecular lipid membrane is described in detail.

摘要

通过将一个小的光化光斑移动穿过脂质双分子层含细菌视紫红质重构模型膜的高原-吉布斯边界和双分子部分,研究了脂质双分子层不同部位的光电活性。结果直接证明,掺入脂质膜双分子区域的细菌视紫红质是该系统光电活性的原因。详细描述了一种扫描修饰双分子脂质膜光电活性的技术。

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本文引用的文献

2
Incorporation of bacteriorhodopsin into a bilayer lipid membrane; a photoelectric-spectroscopic study.
FEBS Lett. 1976 Dec 15;72(1):136-8. doi: 10.1016/0014-5793(76)80829-0.
3
Photo-induced potentials across a polymer stabilized planar membrane, in the presence of bacteriorhodopsin.
Biochem Biophys Res Commun. 1976 Jul 26;71(2):603-9. doi: 10.1016/0006-291x(76)90830-5.
4
Direct evidence of charge-induced lipid domain structure in model membranes.
FEBS Lett. 1977 Jun 15;78(2):169-72. doi: 10.1016/0014-5793(77)80298-6.
6
Mechanism of generation and regulation of photopotential by bacteriorhodopsin in bimolecular lipid membrane.
Biochim Biophys Acta. 1978 Aug 8;503(2):304-15. doi: 10.1016/0005-2728(78)90190-1.

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