平面膜中细菌视紫红质对光的电响应。
The electrical response to light of bacteriorhodopsin in planar membranes.
作者信息
Herrmann T R, Rayfield G W
出版信息
Biophys J. 1978 Feb;21(2):111-25. doi: 10.1016/S0006-3495(78)85512-X.
Abstract
We have measured the light-induced short-circuit current generated by a planar membrane containing bacteriorhodopsin incorporated by vesicle fusion. The experimental results are consistent with an equivalent electrical circuit analogue that assumes that the vesicles remain intact after fusion and that the current generator equivalent of the light-driven proton pump is linearly dependent on bias voltage. The transient response to light of the planar membrane has also been examined. Slow response times are seen to be associated with the capacitive charging and discharging of the fused vesicles. A study of the leading edge of the light response curve of the planar membrane yields information about the transient response of the light-driven proton pump. We propose that the translocation of protons across the membrane is associated with a first-order process characterized by a rate constant lambda.
摘要
我们测量了通过囊泡融合掺入细菌视紫红质的平面膜产生的光诱导短路电流。实验结果与等效电路模型一致，该模型假定囊泡在融合后保持完整，并且光驱动质子泵的等效电流发生器与偏置电压呈线性相关。还研究了平面膜对光的瞬态响应。发现缓慢的响应时间与融合囊泡的电容充电和放电有关。对平面膜光响应曲线前沿的研究得出了有关光驱动质子泵瞬态响应的信息。我们提出质子跨膜转运与以速率常数λ为特征的一级过程有关。
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