Likhachev A Ia, Petrov A S, P'rvanova L G, Pozharisskiĭ K M
Biull Eksp Biol Med. 1978 Dec;86(12):679-81.
The effect of disulfiram on the alkylation of purine bases of the liver and colon DNA in rats exposed to 1,2-dimethyl-hydrazine-3H has been studied. The pretreatment of rats with disulfiram which inhibits the DMH N-oxydation was found to prevent the methylation of guanine with DMH. However, the radioactivity was readily incorporated into normal (non-methylated) purine bases during synthesis. The lack of DNA methylation in animals pretreated with disulfiram indicated carbonium ion rather than methyl radical to alkylate DNA.
研究了双硫仑对暴露于1,2-二甲基-3H-肼的大鼠肝脏和结肠DNA嘌呤碱烷基化的影响。发现用抑制DMH N-氧化的双硫仑预处理大鼠可防止DMH使鸟嘌呤甲基化。然而，在合成过程中放射性很容易掺入正常（未甲基化）嘌呤碱中。用双硫仑预处理的动物缺乏DNA甲基化表明碳正离子而非甲基自由基使DNA烷基化。
