Sato E, Goto M, Nakamura T
Gan. 1981 Apr;72(2):245-53.
The nuclear areas, perimeter, diameter and form factor, and nucleolar area and diameter of about 50 cells in each in 38 colonic epithelial lesions including normal epithelium (6), Peutz-Jeghers polyp (2), adenoma (11), dysplasia (6) and carcinoma (13) were measured at the electron microscopic level by using an image analyzer. The area of nucleoli was larger in dysplasia and carcinoma than the normal (statistically significant). The area of nuclei was larger in carcinoma than the normal. The form factor was also largest in the nuclei of carcinoma, indicating that these cells had the most spherical shape. These results suggest the possibility of quantitative diagnosis of epithelial lesions by measuring the area and perimeter of some 50 nuclei and nucleoli.
使用图像分析仪在电子显微镜水平下，对38例结肠上皮病变（包括正常上皮6例、黑斑息肉病息肉2例、腺瘤11例、发育异常6例和癌13例）中每组约50个细胞的核面积、周长、直径和形态因子，以及核仁面积和直径进行了测量。发育异常和癌中的核仁面积比正常情况大（具有统计学意义）。癌中的细胞核面积比正常情况大。形态因子在癌细胞核中也是最大的，表明这些细胞的形状最接近球形。这些结果提示，通过测量约50个细胞核和核仁的面积和周长，对上皮病变进行定量诊断是有可能的。
