从小鼠骨髓中分离低自我更新能力的造血干细胞。
Separation of hemopoietic stem cells of low self-renewal capacity from mouse bone marrow.
作者信息
Mori K J, Seto A
出版信息
Gan. 1981 Apr;72(2):322-6.
PMID:7286554
Abstract
A simple technique to concentrate hemopoietic stem cells (colony-forming unit in spleen, CFUs) on a ficoll-triosil gradient, permitting an enrichment of the stem cells from mouse bone marrow by a factor of 10, was developed. The proportion of the enriched cells engaged in DNA synthesis was shown to be around 40%. The self-renewal capacity of the enriched stem cells was significantly lower than that of the total stem cells in normal mouse bone marrow. The separated stem cells may correspond to CFUs-2.
摘要
开发了一种简单的技术，可在菲可-三硅醇梯度上浓缩造血干细胞（脾集落形成单位，CFUs），从而使小鼠骨髓中的干细胞富集10倍。结果显示，富集细胞中参与DNA合成的比例约为40%。富集干细胞的自我更新能力明显低于正常小鼠骨髓中总干细胞的自我更新能力。分离出的干细胞可能对应于CFUs-2。
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