Rapid analysis of cholesterol of high density lipoprotein and low density lipoprotein in human serum by high performance liquid chromatography.

A simple and rapid method for the determination of cholesterol in human serum lipoproteins (VLDL, LDL, HDL2, and HDL3) was described. The contents of cholesterol of lipoprotein fractions from a very small amount of serum (20 microliter) can be determined by the enzymatic assay after separation by high performance liquid chromatography in less than 50 min. The method is very useful for studying lipoprotein metabolism and related diseases.