• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

高效液相色谱法快速分析人血清中高密度脂蛋白和低密度脂蛋白胆固醇

Rapid analysis of cholesterol of high density lipoprotein and low density lipoprotein in human serum by high performance liquid chromatography.

作者信息

Hara I, Okazaki M, Ohno Y

出版信息

J Biochem. 1980 Jun;87(6):1863-5. doi: 10.1093/oxfordjournals.jbchem.a132933.

DOI:10.1093/oxfordjournals.jbchem.a132933
PMID:7400128
Abstract

A simple and rapid method for the determination of cholesterol in human serum lipoproteins (VLDL, LDL, HDL2, and HDL3) was described. The contents of cholesterol of lipoprotein fractions from a very small amount of serum (20 microliter) can be determined by the enzymatic assay after separation by high performance liquid chromatography in less than 50 min. The method is very useful for studying lipoprotein metabolism and related diseases.

摘要

描述了一种测定人血清脂蛋白（极低密度脂蛋白、低密度脂蛋白、高密度脂蛋白2和高密度脂蛋白3）中胆固醇的简单快速方法。通过高效液相色谱分离后，采用酶法测定，仅需20微升极少量血清中的脂蛋白组分胆固醇含量，耗时不到50分钟。该方法对于研究脂蛋白代谢及相关疾病非常有用。

相似文献

1
Rapid analysis of cholesterol of high density lipoprotein and low density lipoprotein in human serum by high performance liquid chromatography.高效液相色谱法快速分析人血清中高密度脂蛋白和低密度脂蛋白胆固醇
J Biochem. 1980 Jun;87(6):1863-5. doi: 10.1093/oxfordjournals.jbchem.a132933.
2
Rapid method for the quantitation of cholesterol in human serum lipoproteins by high performance liquid chromatography.用高效液相色谱法定量检测人血清脂蛋白中胆固醇的快速方法。
J Biochem. 1981 Mar;89(3):879-87. doi: 10.1093/oxfordjournals.jbchem.a133271.
3
Effects of niceritrol (pentaerythritol tetranicotinate) on plasma lipoprotein concentration: increment of high density lipoprotein(HDL) cholesterol and HDL-cholesterol/low density lipoprotein cholesterol ratio in hypo-high density lipoproteinemia.烟浪丁（季戊四醇四烟酸酯）对血浆脂蛋白浓度的影响：低高密度脂蛋白血症中高密度脂蛋白（HDL）胆固醇及HDL胆固醇/低密度脂蛋白胆固醇比值的升高
Artery. 1982;10(4):266-85.
4
High-performance aqueous gel permeation chromatography of serum lipoproteins: selective detection of cholesterol by enzymatic reaction.血清脂蛋白的高效水相凝胶渗透色谱法：通过酶促反应选择性检测胆固醇
J Chromatogr. 1981 May 8;223(2):285-93. doi: 10.1016/s0378-4347(00)80100-0.
5
Analyzing of high-density lipoprotein subfractions and low-density lipoprotein subfractions in human serum with anion-exchange chromatography.采用阴离子交换色谱法分析人血清中的高密度脂蛋白亚组分和低密度脂蛋白亚组分。
Atherosclerosis. 2009 Jun;204(2):e52-7. doi: 10.1016/j.atherosclerosis.2008.10.031. Epub 2008 Nov 8.
6
Fractionation of human serum lipoproteins by high performance liquid chromatography. I.用高效液相色谱法分离人血清脂蛋白。I.
J Biochem. 1981 Jun;89(6):1675-80. doi: 10.1093/oxfordjournals.jbchem.a133367.
7
Identification of unique lipoprotein subclasses for visceral obesity by component analysis of cholesterol profile in high-performance liquid chromatography.通过高效液相色谱法中胆固醇谱的成分分析鉴定内脏肥胖的独特脂蛋白亚类。
Arterioscler Thromb Vasc Biol. 2005 Mar;25(3):578-84. doi: 10.1161/01.ATV.0000155017.60171.88. Epub 2005 Jan 6.
8
Role and metabolism of high density lipoprotein subfractions--analysis of serum HDL2-cholesterol and HDL3-cholesterol in patients with various diseases by high performance liquid chromatography.高密度脂蛋白亚组分的作用与代谢——应用高效液相色谱法分析各类疾病患者血清中的高密度脂蛋白2胆固醇和高密度脂蛋白3胆固醇
Bull Tokyo Med Dent Univ. 1982 Dec;29(4):131-7.
9
High-density lipoprotein subpopulations as substrates for the transfer of cholesteryl esters to very-low-density lipoproteins.高密度脂蛋白亚群作为胆固醇酯向极低密度脂蛋白转移的底物。
Biochem J. 1990 Sep 1;270(2):441-9. doi: 10.1042/bj2700441.
10
Association of cholesterol concentrations in low-density lipoprotein, high-density lipoprotein, and high-density lipoprotein subfractions, and of apolipoproteins AI and AII, with coronary stenosis and left ventricular function.低密度脂蛋白、高密度脂蛋白及其亚组分中的胆固醇浓度，以及载脂蛋白AI和AII与冠状动脉狭窄和左心室功能的关联。
J Lab Clin Med. 1987 Jan;109(1):19-26.

引用本文的文献

1
Distinct Differences in Lipoprotein Particle Number Evaluation between GP-HPLC and NMR: Analysis in Dyslipidemic Patients Administered a Selective PPARα Modulator, Pemafibrate.脂蛋白颗粒数在 GP-HPLC 和 NMR 检测中的差异：在给予选择性过氧化物酶体增殖物激活受体α调节剂（Pemafibrate）的血脂异常患者中的分析。
J Atheroscler Thromb. 2021 Sep 1;28(9):974-996. doi: 10.5551/jat.60764. Epub 2021 Feb 2.
2
Hyphenating size-exclusion chromatography with electrospray mass spectrometry; using on-line liquid-liquid extraction to study the lipid composition of lipoprotein particles.将尺寸排阻色谱与电喷雾质谱联用；采用在线液-液萃取法研究脂蛋白颗粒的脂质组成。
Rapid Commun Mass Spectrom. 2015 Nov 15;29(21):1969-76. doi: 10.1002/rcm.7301.
3
Subfractionation and characterization of native and incubation enlarged human plasma high density lipoprotein particles by high performance gel filtration.
通过高效凝胶过滤对天然和孵育后增大的人血浆高密度脂蛋白颗粒进行亚组分分离和表征。
Lipids. 1985 Jun;20(6):378-88. doi: 10.1007/BF02534206.