Ariga T, Yu R K
J Lipid Res. 1987 Mar;28(3):285-91.
A sialidase-susceptible monosialoganglioside was isolated from normal human brain by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The yield of this ganglioside was about 6 mg per whole brain. Its structure was elucidated by sugar analysis, sialidase digestion, permethylation, and proton NMR studies. This ganglioside had carbohydrate, fatty acid, and long-chain base compositions identical to those of brain GM1a. However, the sialosyl residue was found to be linked (alpha 2-3) to the terminal galactosyl residue of the asialo-GM1a backbone. The complete structure of this ganglioside was therefore identified as GM1b or IV3NeuAcGgOse4Cer.
通过DEAE - 葡聚糖凝胶A - 25和离子交换树脂柱色谱法从正常人脑中分离出一种对唾液酸酶敏感的单唾液酸神经节苷脂。该神经节苷脂的产量约为每整个大脑6毫克。通过糖分析、唾液酸酶消化、全甲基化和质子核磁共振研究阐明了其结构。这种神经节苷脂的碳水化合物、脂肪酸和长链碱基组成与脑GM1a相同。然而，发现唾液酸残基以（α2 - 3）连接到脱唾液酸GM1a主链的末端半乳糖残基上。因此，这种神经节苷脂的完整结构被鉴定为GM1b或IV3NeuAcGgOse4Cer。
