De Blas A L, Ratnaparkhi M V, Mosimann J E
J Immunol Methods. 1981;45(2):109-15. doi: 10.1016/0022-1759(81)90205-2.
A practical method is described for the estimation of the number of monoclonal hybridomas in a cell fusion experiment as a function of the percent of culture dishes showing hybridoma growth. Our method is based on the Poisson probability model. A justification for the method is included. The application of this model to our experimental results indicates that the probability of hybridoma survival decreases with post-fusion cell dilution even in the presence of a constant number of feeder cells.
本文描述了一种实用方法，用于估算细胞融合实验中单个克隆杂交瘤的数量，该数量是显示杂交瘤生长的培养皿百分比的函数。我们的方法基于泊松概率模型。文中还包括了该方法的合理性说明。将此模型应用于我们的实验结果表明，即使存在恒定数量的饲养细胞，杂交瘤存活的概率也会随着融合后细胞稀释而降低。
