McKeown M, Taylor W C, Kindle K L, Firtel R A, Bender W, Davidson N
Cell. 1978 Nov;15(3):789-800. doi: 10.1016/0092-8674(78)90264-7.
We have used an actin gene-containing restriction fragment of plasmid M6 (Kindle and Firtel, 1978) to select a second actin gene-containing plasmid which we have named pDd actin 2. This plasmid has been shown to contain two actin genes separated by 350 bp of nonactin DNA. When heteroduplexes are formed between any two of the three actin genes present in chimeric plasmids, the region of homology is 1100 +/- 100 bp. This is close to the minimum length required to code for actin protein. The 1100 bp region of intergene homology corresponds to the 1100 bp homology observed between M6 and the two actin cDNA plasmids pcDd actin B1 and pcDd actin A1 (Bender et al., 1978). We have no evidence for additional sequences common to either the 3' or 5' ends of the 1100 +/- 100 bp region of intergene homology. Thermal denaturation experiments show that different pairs of actin genes are diverged from each other by as much as 6--8%. There are two size classes of mRNA complementary to the three actin genes. These have lenghts of 1.25 and 1.35 kb as determined on methyl mercuric hydroxide-containing agarose gels. The possible linkage of these three actin genes to other actin genes is discussed.
我们利用质粒M6中含肌动蛋白基因的限制性片段（Kindle和Firtel，1978年）筛选出了另一个含肌动蛋白基因的质粒，我们将其命名为pDd肌动蛋白2。已证明该质粒含有两个肌动蛋白基因，它们被350 bp的非肌动蛋白DNA隔开。当嵌合质粒中存在的三个肌动蛋白基因中的任意两个形成异源双链体时，同源区域为1100±100 bp。这接近编码肌动蛋白所需的最小长度。基因间同源性的1100 bp区域与M6和两个肌动蛋白cDNA质粒pcDd肌动蛋白B1和pcDd肌动蛋白A1之间观察到的1100 bp同源性相对应（Bender等人，1978年）。我们没有证据表明基因间同源性的1100±100 bp区域的3'或5'端存在其他共同序列。热变性实验表明，不同的肌动蛋白基因对彼此的差异高达6%-8%。有两种大小类别的mRNA与这三个肌动蛋白基因互补。在含甲基汞的琼脂糖凝胶上测定，它们的长度分别为1.25 kb和1.35 kb。讨论了这三个肌动蛋白基因与其他肌动蛋白基因可能的连锁关系。
