Stemerman M B
Arteriosclerosis. 1981 Jan-Feb;1(1):25-32. doi: 10.1161/01.atv.1.1.25.
We investigated the effects of moderate hypercholesterolemia and endothelial regrowth on the function of vascular endothelium. Groups of rabbits were fed either a high or low cholesterol diet and subjected to removal of the aortic endothelium or sham-operated. Endothelial removal caused diffuse thickening of the myointima. The high cholesterol diet did not affect the extent of endothelial regeneration. To test endothelial function, 1 minute before sacrifice each animal was infused with horseradish peroxidase, which will penetrate the intima in areas of increased permeability. After sacrifice, the aorta was removed en bloc and exposed to diaminobenzidine and H2O2, which produce a brown stain on reaction with horseradish peroxidase. The luminal surface of the aorta was examined, and brown-stained areas, indicating increased permeability, were quantitated by stereology. Rabbits fed the cholesterol-rich diet had a greater percentage of brown-stained areas than did rabbits fed the low cholesterol diet; this was true for both intact and regenerated endothelium. The differences were significant at the p less than 0.01 level. Scanning electron microscopy of brown areas showed that, although the surface characteristics of the endothelium were altered, endothelial sloughing did not occur. Therefore, it appears that hypercholesterolemia can be associated with a functional endothelial defect in the absence of endothelial loss.
我们研究了中度高胆固醇血症和内皮再生长对血管内皮功能的影响。将兔分为几组，分别给予高胆固醇或低胆固醇饮食，并进行主动脉内皮剥脱或假手术。内皮剥脱导致肌内膜弥漫性增厚。高胆固醇饮食不影响内皮再生的程度。为了检测内皮功能，在处死前1分钟给每只动物输注辣根过氧化物酶，该酶会渗透到通透性增加区域的内膜中。处死动物后，将主动脉整体取出，暴露于二氨基联苯胺和过氧化氢中，它们与辣根过氧化物酶反应会产生棕色染色。检查主动脉的管腔表面，并用体视学方法对指示通透性增加的棕色染色区域进行定量。喂食富含胆固醇饮食的兔的棕色染色区域百分比高于喂食低胆固醇饮食的兔；完整内皮和再生内皮的情况均如此。差异在p小于0.01水平时具有显著性。对棕色区域进行扫描电子显微镜检查显示，尽管内皮的表面特征发生了改变，但未发生内皮脱落。因此，似乎在没有内皮丢失的情况下，高胆固醇血症可伴有功能性内皮缺陷。
