人类皮肤中的环氧水解酶活性。
Epoxide hydrolase activity in human skin.
作者信息
O'Neill V A, Rawlins M D, Chapman P H
出版信息
Br J Clin Pharmacol. 1981 Oct;12(4):517-21. doi: 10.1111/j.1365-2125.1981.tb01260.x.
Abstract
1 Epoxide hydrolase (EH) activity was measured in biopsied skin (n = 42) using 7-[H3]-styrene oxide as substrate, and separation of the products by high performance liquid chromatography. 2 EH activity (mean +/- s.d.) was present in separated epidermis (139 +/- 105 pmol glycol formed mg-1 min-1) and dermis (165 +/- 120 pmol glycol formed mg-1 microsomal protein min-1). 3 Whole skin EH activity (mean +/- s.d.) varied widely (433 +/- 254 pmol glycol formed mg-1 microsomal protein min-1) 4 No significant difference in EH activity was observed in skin from breast, penis and leg. 5 Skin EH activity does not appear to contribute significantly to the systemic metabolism of epoxide, but may be important in determining the effects of epoxides formed within the epidermis.
摘要
- 使用7-[H3]-氧化苯乙烯作为底物，通过高效液相色谱法分离产物，测定了42例活检皮肤样本中的环氧水解酶（EH）活性。2. 分离的表皮（形成139±105 pmol二醇mg-1 min-1）和真皮（形成165±120 pmol二醇mg-1微粒体蛋白min-1）中存在EH活性（平均值±标准差）。3. 全皮EH活性（平均值±标准差）差异很大（形成433±254 pmol二醇mg-1微粒体蛋白min-1）。4. 在乳房、阴茎和腿部皮肤中未观察到EH活性的显著差异。5. 皮肤EH活性似乎对环氧化物的全身代谢没有显著贡献，但可能在确定表皮内形成的环氧化物的作用方面很重要。
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