Thielmann K, Rühling K, Schauer I, Leonhardt I, Herzberg V
Clin Chim Acta. 1981 Sep;115(3):297-309. doi: 10.1016/0009-8981(81)90243-6.
Enzymatic methods adapted to the KAPA system can be carried out in a final test volume of less than 10 mu 1. The procedure is mechanized, including the pre-analytical steps. Day-to-day precision is 2-6% C.V. for single determinations. Advantages of the technique are: (1) capillary plasma can be used; (2) reagent waste is reduced by about two orders of magnitude compared to common procedures, and (3) 400 specimens can be assayed by one technician per day. These conditions facilitate serial determinations, large scale screening, or repetitive monitoring of values in a group of individuals.
适用于KAPA系统的酶法可在最终测试体积小于10微升的情况下进行。该程序实现了机械化,包括分析前步骤。单次测定的日常精密度为2-6%变异系数。该技术的优点包括:(1)可使用毛细血管血浆;(2)与常规程序相比,试剂浪费减少了约两个数量级;(3)一名技术人员每天可检测400个样本。这些条件便于对一组个体进行系列测定、大规模筛查或重复监测数值。