The use of combustion technique of tissue processing for the study of the distribution of 14C-salicylate in rats after oral administration.

The distribution of 14C-salicylate (150 mg/kg, orally) in rat tissues was studied, at different time intervals upto 2 hr. A fairly rapid distribution was noted and a peak level was found to occur at 30 min after administration of the labelled drug in all the tissue studied. The combustion technique was used for tissue processing to determine the radioactivity as compared to the digestion method of tissue processing utilised by Sturman et al. (5) for their studies. The combustion technique was found to be more sensitive, as at a dose of 150 mg/kg (0.5 ml containing 5 uCi) administered orally, there was a greater level of radioactivity in all tissues as compared to the levels found by Sturman et al. (5) for a dose of 200 mg/kg (0.2 ml containing 2 uCi) administered intraperitoneally.