Anastassiades T, Puzic R, Puzic O
J Chromatogr. 1981 Oct 9;225(2):309-18.
A modification of a gas--liquid chromatographic method is described that allows better simultaneous separations of the neutral and aminosugar alditol acetate derivatives as single peaks. using 3% SP-2340 on 100-200 mesh Supelcoport, retention times were relatively short and baseline separation between glucose and galactose was achieved. The method is particularly suitable for monitoring the fractionation of complex mixtures of glycoproteins and glycosaminoglycans, and its application is illustrated in the fractionation of bone matrix extracts subjected to ion-exchange chromatography. A convenient procedure allowing the separation and estimation of sialic acid in the same aliquot is also described and evaluated.
本文描述了一种气相色谱法的改进方法，该方法能更好地将中性糖和氨基糖糖醇乙酸酯衍生物同时分离为单峰。使用涂有3% SP - 2340的100 - 200目Supelcoport，保留时间相对较短，实现了葡萄糖和半乳糖之间的基线分离。该方法特别适用于监测糖蛋白和糖胺聚糖复杂混合物的分级分离，其应用在经离子交换色谱法处理的骨基质提取物的分级分离中得到了说明。还描述并评估了一种方便的程序，该程序可在同一等分试样中分离和估算唾液酸。
