人II组胃蛋白酶原的放射免疫定量分析

Radioimmunological quantitation of human group-II pepsinogens.

作者信息

Matzku S, Zöller M, Rapp W

出版信息

Digestion. 1978;18(1-2):16-26. doi: 10.1159/000198180.

DOI:10.1159/000198180

Abstract

A solid-phase sandwich radioimmunoassay was developed to quantitate human group-II pepsinogens in plasma. The test detected pepsinogen II in a concentration range of 0.25--64.0 ng/ml using sample volumes of 125 microliter. Purified group I pepsinogens showed no response up to concentrations of 100 microgram/ml. In apparently healthy donors, we observed mean plasma concentrations of 20.3 ng/ml (males) and of 15.5 ng/ml (females).

摘要

已开发出一种固相夹心放射免疫分析法，用于定量检测血浆中的人II组胃蛋白酶原。该检测方法使用125微升的样本体积，可检测浓度范围为0.25 - 64.0纳克/毫升的胃蛋白酶原II。纯化的I组胃蛋白酶原在浓度高达100微克/毫升时无反应。在明显健康的供体中，我们观察到男性血浆平均浓度为20.3纳克/毫升，女性为15.5纳克/毫升。