I组胃蛋白酶原（PGI）的放射免疫测定及食物对血清PGI的影响。

Radioimmunoassay of group I pepsinogens (PGI) and the effect of food on serum PGI.

作者信息

Waldum H L, Straume B K, Burhol P G

出版信息

Scand J Gastroenterol. 1979;14(2):241-7. doi: 10.3109/00365527909179877.

Abstract

A sensitive and precise radioimmunoassay of group I pepsinogens (PGI) in serum is described. The method is a modification of the one described by Samloff and Liebman. The most important improvement is that 125I-labeled PGI can be stored diluted for at least 7 weeks without loss of immunoreactivity. The present method has a detection limit of 2.6 ng/ml, a within-assay precision of 6%, and a between-assay precision of 17% in the normal range. A light test meal had no significant effect on serum PGI. Serum PGI in 388 non-fasting subjects 20-49 years old was 164 +/- 5 ng/ml (mean +/- S.E.M.). In these subjects serum PGI was significantly higher in men than in women. In both sexes the frequency distribution showed two peaks, indicating the presence of two different populations.

摘要

本文描述了一种灵敏且精确的血清I型胃蛋白酶原（PGI）放射免疫测定法。该方法是对Samloff和Liebman所描述方法的改进。最重要的改进是，125I标记的PGI稀释后可保存至少7周而不丧失免疫反应性。本方法的检测限为2.6 ng/ml，在正常范围内批内精密度为6%，批间精密度为17%。清淡试餐对血清PGI无显著影响。388名20 - 49岁非空腹受试者的血清PGI为164±5 ng/ml（均值±标准误）。在这些受试者中，男性血清PGI显著高于女性。在两性中，频率分布均显示两个峰值，表明存在两个不同的群体。