Ts'ao C, Eisenstein R
Lab Invest. 1981 Nov;45(5):450-5.
We attached bovine aorta and cartilage proteoglycan fractions to extracted human skin collagen and studied the effect of such treatment on the platelet-aggregating activity of collagen. Attachment of bovine aorta and cartilage proteoglycan fractions to collagen was confirmed by electron microscopy and uronic acid analyses, Collagen attached with aorta proteoglycan retained its aggregating activity; collagen attached with cartilage proteoglycan caused a delayed platelet aggregation response. However, platelets preincubated with cartilage proteoglycan proceeded to normal aggregation when challenged by untreated collagen. Neither proteoglycan affected platelet aggregation induced by ADP. Preincubation of collagen with aorta proteoglycan did not protect the collagen from being inactivated by plasma. Of the proteoglycan fractions tested, only those from aorta were antithrombic and the antithrombic activity was plasma dependent.
我们将牛主动脉和软骨蛋白聚糖组分附着于提取的人皮肤胶原蛋白上，并研究了这种处理对胶原蛋白血小板聚集活性的影响。通过电子显微镜和糖醛酸分析证实了牛主动脉和软骨蛋白聚糖组分与胶原蛋白的附着。附着有主动脉蛋白聚糖的胶原蛋白保留了其聚集活性；附着有软骨蛋白聚糖的胶原蛋白引起血小板聚集反应延迟。然而，用软骨蛋白聚糖预孵育的血小板在受到未处理的胶原蛋白刺激时会进行正常聚集。两种蛋白聚糖均不影响ADP诱导的血小板聚集。用主动脉蛋白聚糖预孵育胶原蛋白并不能保护胶原蛋白不被血浆灭活。在所测试的蛋白聚糖组分中，只有来自主动脉的那些具有抗血栓形成作用，且抗血栓形成活性依赖于血浆。
