不同年龄小鼠组织中转录RNA水平及合成速率的变化

Changes in the levels and the rate of synthesis of transfer RNA in tissues of mice of different ages.

作者信息

Neumeister J A, Webster G C

出版信息

Mech Ageing Dev. 1981;16(4):319-26. doi: 10.1016/0047-6374(81)90016-6.

Abstract

Levels of transfer RNA (tRNA) were determined in liver, kidney, skeletal muscle, heart, and brain of young (35-day), adult (12-month) and old (24-month) female C57BL/6J mice. Kidney and liver showed little change in tRNA levels between young and adult mice, but the levels decreased in old mice. Skeletal muscle tRNA decreased steadily from young to old mice. Heart tRNA increased during maturation to adult organisms and then decreased in old individuals. Brain levels of tRNA increased steadily. No age-related change in the rate of transport of orotic acid into cells was observed. However, all tissues exhibited a decrease in uridine pools between adult and old mice. Most importantly, all tissues of aging mice showed a decrease in the rate of tRNA synthesis.

摘要

在年轻（35天）、成年（12个月）和老年（24个月）雌性C57BL/6J小鼠的肝脏、肾脏、骨骼肌、心脏和大脑中测定了转运RNA（tRNA）的水平。肾脏和肝脏在年轻和成年小鼠之间tRNA水平变化不大，但在老年小鼠中水平下降。从年轻到老年小鼠，骨骼肌tRNA稳步下降。心脏tRNA在成熟到成年生物体的过程中增加，然后在老年个体中下降。大脑tRNA水平稳步上升。未观察到乳清酸进入细胞的运输速率与年龄相关的变化。然而，成年和老年小鼠之间所有组织的尿苷池均减少。最重要的是，衰老小鼠的所有组织均显示tRNA合成速率下降。