衰老小鼠不同器官的无细胞制剂蛋白质合成速率降低。

Decreased rates of protein synthesis by cell-free preparations from different organs of aging mice.

作者信息

Blazejowski C A, Webster G C

出版信息

Mech Ageing Dev. 1983 Mar-Apr;21(3-4):345-56. doi: 10.1016/0047-6374(83)90051-9.

Abstract

Protein synthetic activity was determined in postmitochondrial preparations from heart, brain, kidney, liver and skeletal muscle of 5-26-month-old female C57B1/6J mice. An age-dependent decrease in the rate of protein synthesis was exhibited by all preparations except heart muscle. A 65% decrease in translational rate was found in liver, with the greatest decrease appearing after 21 months. Translation in the brain preparation declined little during the first 20 months, but dropped 33% between 20 and 26 months. The kidney preparation decreased 30% during the first 16 months and 70% by the end of 26 months of age. Skeletal muscle showed an overall decrease of 85% in translation rate. In contrast, heart muscle decreased no more than 10% over the life-span of the mice. From these results, it appears that aging has a differential effect on protein synthesis in different kinds of cells.

摘要

在5至26月龄雌性C57B1/6J小鼠的心脏、大脑、肾脏、肝脏和骨骼肌的线粒体后提取物中测定蛋白质合成活性。除心肌外，所有提取物均表现出蛋白质合成速率随年龄增长而下降。肝脏中的翻译速率下降了65%，在21个月后下降幅度最大。在前20个月，大脑提取物中的翻译几乎没有下降，但在20至26个月之间下降了33%。肾脏提取物在最初16个月内下降了30%，到26月龄结束时下降了70%。骨骼肌的翻译速率总体下降了85%。相比之下，在小鼠的整个生命周期中，心肌的下降不超过10%。从这些结果来看，衰老对不同类型细胞中的蛋白质合成具有不同的影响。