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微型μ和微型D108的物理特性

Physical characterization of mini-mu and mini-D108.

作者信息

Résibois A, Toussaint A, van Gijsegem F, Faelen M

出版信息

Gene. 1981 Jun-Jul;14(1-2):103-13. doi: 10.1016/0378-1119(81)90152-9.

DOI:10.1016/0378-1119(81)90152-9
PMID:6266926
Abstract

Several derivatives of phages Mu and D108 have been isolated that carry an internal deletion generated by one of the IS1 components of a Tn9 transposon located in the A, B, or S gene of the prenatal phage. The deletions remove most of the lytic functions of the phage but leave intact either genes A and B or gene A and the left and the right end of the phages. These deleted derivatives, called mini-Mu and mini-D108, were physically characterized by electron microscopy and digestion with restriction enzymes. Mini-Mu and mini-D108, which carry an antibiotic resistance marker, are described and some of their genetic properties are summarized in the paper by Toussaint et al. (1981).

摘要

已经分离出噬菌体Mu和D108的几种衍生物,它们带有由位于前噬菌体A、B或S基因中的Tn9转座子的一个IS1元件产生的内部缺失。这些缺失去除了噬菌体的大部分裂解功能,但使基因A和B或基因A以及噬菌体的左端和右端保持完整。这些缺失的衍生物,称为微型Mu和微型D108,通过电子显微镜和限制性酶消化进行了物理表征。携带抗生素抗性标记的微型Mu和微型D108在图森特等人(1981年)的论文中进行了描述,并总结了它们的一些遗传特性。

相似文献

1
Physical characterization of mini-mu and mini-D108.微型μ和微型D108的物理特性
Gene. 1981 Jun-Jul;14(1-2):103-13. doi: 10.1016/0378-1119(81)90152-9.
2
The products of gene A of the related phages Mu and D108 differ in their specificities.相关噬菌体Mu和D108的基因A产物在特异性上有所不同。
Mol Gen Genet. 1983;190(1):70-9. doi: 10.1007/BF00330326.
3
Mutator bacteriophage D108 and its DNA: an electron microscopic characterization.诱变噬菌体D108及其DNA:电子显微镜表征
J Virol. 1981 Jan;37(1):420-30. doi: 10.1128/JVI.37.1.420-430.1981.
4
Comparison of left-end DNA sequences of bacteriophages Mu and D108.
Gene. 1985;33(2):235-9. doi: 10.1016/0378-1119(85)90098-8.
5
The right end of transposable bacteriophage D108 contains a 520 base pair protein-encoding sequence not present in bacteriophage Mu.可转座噬菌体D108的右端包含一段520个碱基对的蛋白质编码序列,该序列在噬菌体Mu中不存在。
Nucleic Acids Res. 1987 Aug 25;15(16):6691-704. doi: 10.1093/nar/15.16.6691.
6
Genetic characterization of Mu-like bacteriophage D108.类Mu噬菌体D108的遗传特征分析
J Virol. 1978 Sep;27(3):513-8. doi: 10.1128/JVI.27.3.513-518.1978.
7
Chromosomal rearrangements induced by mini-Mu and mini-D108: mini review and new data.由微型Mu和微型D108诱导的染色体重排:综述与新数据
Gene. 1981 Jun-Jul;14(1-2):115-9. doi: 10.1016/0378-1119(81)90153-0.
8
Characterization of the lysogenic repressor (c) from transposable Mu-like bacteriophage D108.可转座的类Mu噬菌体D108溶原性阻遏物(c)的特性分析
Nucleic Acids Res. 1991 Nov 11;19(21):5949-56. doi: 10.1093/nar/19.21.5949.
9
Bacteriophage Mu sites and functions involved in the inhibition of lambda::mini-Mu growth.与抑制λ::mini-Mu生长相关的噬菌体Mu位点及功能。
Virology. 1990 Jul;177(1):95-105. doi: 10.1016/0042-6822(90)90463-2.
10
Chromosomal rearrangements by an IS2 insertion in phage Mu-1.噬菌体Mu-1中因IS2插入导致的染色体重排。
Gene. 1978 Sep;4(1):51-68. doi: 10.1016/0378-1119(78)90014-8.

引用本文的文献

1
Interaction of bacteriophage P1 with an epiphytic strain-the role of the interplay between various mobilome elements.噬菌体P1与一种附生菌株的相互作用——各种可移动基因组元件之间相互作用的作用
Front Microbiol. 2024 Mar 25;15:1356206. doi: 10.3389/fmicb.2024.1356206. eCollection 2024.
2
In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi.在体克隆菊欧文氏菌果胶酶和纤维素酶基因。
EMBO J. 1985 Mar;4(3):787-92. doi: 10.1002/j.1460-2075.1985.tb03698.x.
3
Localisation of mini-Mu in its replication intermediates.
微小 Mu 在其复制中间体中的定位。
EMBO J. 1982;1(8):965-9. doi: 10.1002/j.1460-2075.1982.tb01279.x.
4
Identification of a mini-Mu by computer analysis of partially denatured DNA.通过对部分变性DNA进行计算机分析鉴定微小Mu噬菌体
EMBO J. 1982;1(8):959-63. doi: 10.1002/j.1460-2075.1982.tb01278.x.
5
Utilization of plasmid pULB113 (RP4::mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi.利用质粒pULB113(RP4::mini-Mu)构建胡萝卜软腐欧文氏菌菊花亚种的连锁图谱。
J Bacteriol. 1983 Jun;154(3):1489-92. doi: 10.1128/jb.154.3.1489-1492.1983.
6
Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase.菊花欧文氏菌中果胶酶和纤维素酶分泌缺陷的突变体。
J Bacteriol. 1984 Dec;160(3):1199-203. doi: 10.1128/jb.160.3.1199-1203.1984.
7
Mini-Mu-duction as a test for genetic complementation in Escherichia coli.微量转导作为大肠杆菌基因互补性的一种检测方法。
J Bacteriol. 1984 Apr;158(1):357-61. doi: 10.1128/jb.158.1.357-361.1984.
8
The products of gene A of the related phages Mu and D108 differ in their specificities.相关噬菌体Mu和D108的基因A产物在特异性上有所不同。
Mol Gen Genet. 1983;190(1):70-9. doi: 10.1007/BF00330326.
9
Isolation and characterization of Erwinia chrysanthemi mutants defective in degradation of hexuronates.菊欧文氏菌己糖醛酸降解缺陷型突变体的分离与鉴定
J Bacteriol. 1985 Feb;161(2):702-8. doi: 10.1128/jb.161.2.702-708.1985.
10
In vivo mutagenesis of bacteriophage Mu transposase.噬菌体Mu转座酶的体内诱变
J Bacteriol. 1987 Dec;169(12):5700-7. doi: 10.1128/jb.169.12.5700-5707.1987.