Vos J M, Cornelis J J, Limbosch S, Zampetti-Bosseler F, Rommelaere J
Mutat Res. 1981 Sep;83(2):171-8. doi: 10.1016/0027-5107(81)90002-6.
3 hybrid cell lines between mouse fibroblasts (A9) and mouse lymphoma cells (L5178YS) were compared with regard to the ability of UV-pre-irradiated cells to replicate intact (unirradiated) Minute-virus-of-Mice (MVM) and to reactivate UV-irradiated MVM. UV irradiation of cells before virus infection enhanced their ability to plaque intact virus (Enhanced Capacity) to a similar extent in the 3 hybrid cell lines. However, pretreatment of cells with UV radiation enhanced the survival of UV-damaged virus (Enhanced Reactivation) in only 1 of these hybrids. The lack of detectable Enhanced Reactivation in the other hybrids without concomitant change in their Enhanced Capacity, suggests that these processes are at least partly independent. Virus survival in unirradiated cells was similar for all 3 hybrid cell lines, indicating that the absence of detectable Enhanced Reactivation in 2 of the hybrids was not due to the constitutive expression of this process, but might rather result from its loss or extinction. The expression of both Enhanced Capacity and Enhanced Reactivation requires synthesis of protein de novo shortly after cell irradiation.
对3种小鼠成纤维细胞（A9）和小鼠淋巴瘤细胞（L5178YS）之间的杂交细胞系进行了比较，比较了紫外线预照射细胞复制完整（未照射）小鼠微小病毒（MVM）以及重新激活紫外线照射的MVM的能力。在病毒感染前对细胞进行紫外线照射，在这3种杂交细胞系中，细胞形成完整病毒噬斑的能力（增强能力）提高到了相似的程度。然而，用紫外线辐射预处理细胞，仅在其中1种杂交细胞系中提高了紫外线损伤病毒的存活率（增强复活）。在其他杂交细胞系中缺乏可检测到的增强复活，同时其增强能力没有伴随变化，这表明这些过程至少部分是独立的。所有3种杂交细胞系在未照射细胞中的病毒存活率相似，这表明2种杂交细胞系中缺乏可检测到的增强复活并非由于该过程的组成型表达，而是可能由于其丧失或消失。增强能力和增强复活的表达都需要在细胞照射后不久重新合成蛋白质。
