Piperakis S M, McLennan A G
Br J Cancer. 1984 Feb;49(2):199-205. doi: 10.1038/bjc.1984.32.
The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a delay of 36 h between heating and infection. The induction process requires protein synthesis only during the 3 h period immediately following heating; cycloheximide does not prevent the expression of enhanced reactivation if added to the cells after this time. Heat-enhanced reactivation exhibits properties similar in some respects to radiation-enhanced reactivation and indicates an increased capacity of the heated cells to tolerate DNA damage.
如果在感染前对HeLa细胞进行短暂的热休克处理，紫外线照射的腺病毒2在这些细胞中的复活率会提高8至9倍。通过在45.5摄氏度下加热10分钟，并在加热和感染之间延迟36小时，可实现最大程度的复活。诱导过程仅在加热后的紧接3小时内需要蛋白质合成；如果在此时间之后将环己酰亚胺添加到细胞中，它不会阻止增强复活的表达。热增强复活在某些方面表现出与辐射增强复活相似的特性，表明加热后的细胞耐受DNA损伤的能力增强。
