热疗可增强HeLa细胞中受辐照腺病毒的再激活。
Hyperthermia enhances the reactivation of irradiated adenovirus in HeLa cells.
作者信息
Piperakis S M, McLennan A G
出版信息
Br J Cancer. 1984 Feb;49(2):199-205. doi: 10.1038/bjc.1984.32.
Abstract
The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a delay of 36 h between heating and infection. The induction process requires protein synthesis only during the 3 h period immediately following heating; cycloheximide does not prevent the expression of enhanced reactivation if added to the cells after this time. Heat-enhanced reactivation exhibits properties similar in some respects to radiation-enhanced reactivation and indicates an increased capacity of the heated cells to tolerate DNA damage.
摘要
如果在感染前对HeLa细胞进行短暂的热休克处理，紫外线照射的腺病毒2在这些细胞中的复活率会提高8至9倍。通过在45.5摄氏度下加热10分钟，并在加热和感染之间延迟36小时，可实现最大程度的复活。诱导过程仅在加热后的紧接3小时内需要蛋白质合成；如果在此时间之后将环己酰亚胺添加到细胞中，它不会阻止增强复活的表达。热增强复活在某些方面表现出与辐射增强复活相似的特性，表明加热后的细胞耐受DNA损伤的能力增强。
相似文献
1
Hyperthermia enhances the reactivation of irradiated adenovirus in HeLa cells.热疗可增强HeLa细胞中受辐照腺病毒的再激活。
Br J Cancer. 1984 Feb;49(2):199-205. doi: 10.1038/bjc.1984.32.
2
Depression of hyperthermic potentiation in cell killing of ultraviolet light-irradiated Dictyostelium discoideum by pre-heat treatment.通过预热处理降低紫外线照射的盘基网柄菌细胞杀伤中超热增强作用
Int J Radiat Biol. 1989 Aug;56(2):193-9. doi: 10.1080/09553008914551341.
3
Enhanced reactivation of UV-irradiated adenovirus 2 in HeLa cells treated with non-mutagenic chemical agents.在用非诱变化学试剂处理的HeLa细胞中紫外线照射的腺病毒2的增强再激活。
Mutat Res. 1985 Mar;142(3):83-5. doi: 10.1016/0165-7992(85)90044-2.
4
U.V. enhanced reactivation of U.V.-and gamma-irradiated adenovirus in normal human fibroblasts.紫外线增强紫外线和γ射线照射的腺病毒在正常人成纤维细胞中的再激活。
Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Jun;43(6):599-623. doi: 10.1080/09553008314550721.
5
The two inducible responses, SOS and heat-shock, in Escherichia coli act synergistically during Weigle reactivation of the bacteriophage phiX174.在噬菌体φX174的韦格勒再活化过程中，大肠杆菌中的两种诱导性反应，即SOS反应和热休克反应，协同发挥作用。
Int J Radiat Biol. 2007 Jul;83(7):463-9. doi: 10.1080/09553000701371389.
6
Analysis of DNA repair in XP-HeLa hybrids; lack of correlation between excision repair of u.v. damage and adenovirus reactivation in an XP(D)-like cell line.对XP-HeLa杂交细胞中DNA修复的分析；在一种类似XP(D)的细胞系中，紫外线损伤的切除修复与腺病毒再激活之间缺乏相关性。
Carcinogenesis. 1986 Oct;7(10):1733-8. doi: 10.1093/carcin/7.10.1733.
7
Delayed expression of enhanced reactivation and decreased mutagenesis of UV-irradiated adenovirus in UV-irradiated ataxia telangiectasia fibroblasts.紫外线照射的共济失调毛细血管扩张症成纤维细胞中紫外线照射的腺病毒再激活增强和诱变减少的延迟表达
Mutagenesis. 1988 Sep;3(5):389-95. doi: 10.1093/mutage/3.5.389.
8
Heavy metals enhance the reactivation of nitrous acid-treated adenovirus in HeLa cells.重金属可增强亚硝酸处理的腺病毒在HeLa细胞中的复活。
Mutat Res. 1995 Jan;346(1):15-8. doi: 10.1016/0165-7992(95)90063-2.
9
Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ultraviolet light.紫外线照射后人类12型腺病毒和猿猴病毒40的多重复活
Virology. 1971 Aug;45(2):529-31. doi: 10.1016/0042-6822(71)90355-2.
10
Hyperthermia and host-cell reactivation of adenovirus 12.腺病毒12的热疗与宿主细胞复活
Can J Genet Cytol. 1978 Mar;20(1):35-40. doi: 10.1139/g78-006.
引用本文的文献
1
Genetic and environmental factors in polymyalgia rheumatica.风湿性多肌痛中的遗传和环境因素。
Ann Rheum Dis. 1997 Oct;56(10):576-7. doi: 10.1136/ard.56.10.576.
本文引用的文献
1
UV-irradiation of related mouse hybrid cells: similar increase in capacity to replicate intact minute-virus-of-mice but differential enhancement of survival of UV-irradiated virus.相关小鼠杂交细胞的紫外线照射：复制完整小鼠微小病毒的能力有类似增加，但紫外线照射病毒存活的差异增强。
Mutat Res. 1981 Sep;83(2):171-8. doi: 10.1016/0027-5107(81)90002-6.
2
UV-induced reversion of adenovirus 5ts2 infecting human cells.紫外线诱导感染人类细胞的腺病毒5ts2发生回复突变。
Photochem Photobiol. 1981 Sep;34(3):403-6. doi: 10.1111/j.1751-1097.1981.tb09015.x.
3
The pattern of protein synthesis induced by heat shock of HeLa cells.由HeLa细胞热休克诱导的蛋白质合成模式。
Eur J Biochem. 1981 Jul;117(2):341-6. doi: 10.1111/j.1432-1033.1981.tb06343.x.
4
Hyperthermia and the cell nucleus.热疗与细胞核
Radiat Res. 1982 Dec;92(3):458-62.
5
Thermotolerance and heat shock proteins in mammalian cells.哺乳动物细胞中的热耐受性与热休克蛋白
Radiat Res. 1982 Dec;92(3):452-7.
6
Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene product.腺病毒E1A基因产物诱导合成一种70,000道尔顿的哺乳动物热休克蛋白。
Cell. 1982 Jul;29(3):913-9. doi: 10.1016/0092-8674(82)90453-6.
7
Effect of hyperthermia on CHO DNA polymerases alpha and beta.热疗对CHO细胞DNA聚合酶α和β的影响。
Radiat Res. 1982 Jan;89(1):134-49.
8
Hyperthermia and the heat-shock proteins of HeLa cells.HeLa细胞的热疗与热休克蛋白
Br J Cancer. 1982 Jun;45(6):953-63. doi: 10.1038/bjc.1982.148.
9
The effects of hyperthermia on DNA replication in HeLa cells.热疗对HeLa细胞DNA复制的影响。
Radiat Res. 1983 Jan;93(1):71-84.
10
Radiation and mutagen inducible mammalian genes.辐射和诱变剂诱导的哺乳动物基因。
Biochimie. 1982 Aug-Sep;64(8-9):707-8. doi: 10.1016/s0300-9084(82)80114-4.
Zotero 插件