Strange R C, Hume R, Eadington D W, Nimmo I A
Pediatr Res. 1981 Nov;15(11):1425-8. doi: 10.1203/00006450-198111000-00008.
Glycocholate binding by plasma proteins and erythrocytes from fetal and adult blood has been studied, and the results have been used to derive a compartmental model of the distribution of glycocholate in blood. This model assumes that glycocholate is distributed between the aqueous phase, albumin binding sites, binding sites on other plasma proteins, and erythrocytes. Whereas glycocholate binding by albumin was saturable [fetal blood: dissociation constant (0.5 mM), concentration of binding sites (11.3 mumoles/g protein); adult blood: dissociation constant (0.42 mM), concentration of binding sites (20.1 mumoles/g protein)], binding by other plasma proteins was not. The association of glycocholate with erythrocytes appeared to be based on partitioning rather than binding of the bile salt to specific sites. The value of the partition coefficient was 6.0. The compartmental model indicates that, in fetal blood, 46% of the glycocholate is in free solution, and 37% is bound to albumin. In blood from adults, the corresponding values are 31% in free solution and 59% bound to albumin.
已对胎儿和成人血液中的血浆蛋白及红细胞与甘氨胆酸盐的结合情况进行了研究，研究结果被用于推导甘氨胆酸盐在血液中分布的房室模型。该模型假定甘氨胆酸盐分布于水相、白蛋白结合位点、其他血浆蛋白上的结合位点以及红细胞之间。白蛋白与甘氨胆酸盐的结合是可饱和的[胎儿血液：解离常数（0.5 mM），结合位点浓度（11.3微摩尔/克蛋白）；成人血液：解离常数（0.42 mM），结合位点浓度（20.1微摩尔/克蛋白）]，而其他血浆蛋白与甘氨胆酸盐的结合则不然。甘氨胆酸盐与红细胞的结合似乎基于分配作用，而非胆盐与特定位点的结合。分配系数的值为6.0。房室模型表明，在胎儿血液中，46%的甘氨胆酸盐处于游离溶液中，37%与白蛋白结合。在成人血液中，相应的值分别为游离溶液中31%，与白蛋白结合59%。
