Schäfer V, Knothe H
Immun Infekt. 1978 Dec;6(6):214-6.
The influence of nutrient-broth (NB), Mueller-Hinton-broth (MHB), isotonic-sensitive-broth (ISB) and tryptone-soya-broth (TSB) with respect to the determination of MIC values of three tetracyclines is shown. A difference of degradation of tetracycline derivates in the four media was found. The degradation in NB after 18 h was negligible, but in MHB after 18 h all the three tetracyclines were inactivated up to 42%. The MIC levels of staphylococcus aureus strains in NB were about twofold lower than those in MHB and TSB. The MIC levels in ISB were even three- to fourfold higher than in NB. From these results sensitivity testing of tetracycline derivates should be carried out with NB.
研究显示了营养肉汤（NB）、Mueller-Hinton肉汤（MHB）、等渗敏感肉汤（ISB）和胰蛋白胨大豆肉汤（TSB）对三种四环素最低抑菌浓度（MIC）值测定的影响。发现四种培养基中四环素衍生物的降解存在差异。NB中18小时后的降解可忽略不计，但MHB中18小时后所有三种四环素的失活率高达42%。金黄色葡萄球菌菌株在NB中的MIC水平比在MHB和TSB中低约两倍。ISB中的MIC水平甚至比NB中高3至4倍。根据这些结果，四环素衍生物的敏感性测试应使用NB进行。
