Costin J D
Immun Infekt. 1978 Dec;6(6):233-9.
The growth of 16 bacterial cultures, which belong to the frequently encountered pathogens, in three different chemically defined fluid media was investigated through turbidity measurements in comparison with Mueller-Hinton-broth. E. coli, Salm. dublin, Salm. typhi-murium, Enterobacter cloacae, Proteus mirabilis, Pseud. aeruginosa and Bac. cereus grew in two of the three tested defined media with approximately the same intensity as in the Mueller-Hinton broth. Staph. aureus grew well only in one of the 3 tested defined media. Listeria monocytogenes, Erysipelothrix rhusiopathiae, Pasteurella multocida, Neisseria gonorrhoeae and Brucella abortus showed only in one of the three tested media a barely descernible growth, whereas Strept. faecalis, Strept. pneumoniae, Strept. pyogenes showed no growth in the three tested defined media after 34 hours of incubation. None of the tested defined media can be used for routine sensitivity testing but the use of two of the three tested defined media as a reference medium for the standardization of the complex sensitivity testing media remains theoretically possible.
通过比浊法测量,研究了16种属于常见病原体的细菌培养物在三种不同化学成分明确的液体培养基中的生长情况,并与穆勒-辛顿肉汤进行比较。大肠杆菌、都柏林沙门氏菌、鼠伤寒沙门氏菌、阴沟肠杆菌、奇异变形杆菌、铜绿假单胞菌和蜡样芽孢杆菌在三种测试的限定培养基中的两种中生长,其强度与穆勒-辛顿肉汤中大致相同。金黄色葡萄球菌仅在三种测试的限定培养基中的一种中生长良好。产单核细胞李斯特菌、猪红斑丹毒丝菌、多杀巴斯德氏菌、淋病奈瑟菌和流产布鲁氏菌仅在三种测试培养基中的一种中显示出几乎难以察觉的生长,而粪肠球菌、肺炎链球菌、化脓性链球菌在培养34小时后在三种测试的限定培养基中均未生长。所测试的限定培养基均不能用于常规药敏试验,但理论上仍有可能将三种测试的限定培养基中的两种用作复杂药敏试验培养基标准化的参考培养基。