Delbeke E, Chambon J, Sicard D, Gauthier M, Vandekerkove M
Ann Microbiol (Paris). 1981 Jul-Aug;132B(1):41-9.
Usual media are not convenient for the determination of minimal inhibitory concentrations (MIC). An agar medium without haemoglobin and seric proteins (ABNG) is proposed. Using the dilution method with less fastidious bacteria, including Neisseria sp., MIC were identical on Mueller-Hinton agar and ABNG. By using diffusion techniques, the correlation was good between MIC and zone diameters; the density of inoculum for gonococci was 2 x 10(6) CFU/ml. Dilution and diffusion techniques were used for penicillin, ampicillin, thiamphenicol, tetracycline and spectinomycin. It appears that the diffusion technique with ABNG medium would simplify the antibiotic sensitivity survey for N. gonorrhoeae, dilution technique remaining the reference method.
常规培养基不便于测定最低抑菌浓度（MIC）。现提出一种不含血红蛋白和血清蛋白的琼脂培养基（ABNG）。对于包括奈瑟菌属在内的不那么苛求的细菌，采用稀释法时，在穆勒-欣顿琼脂和ABNG上测得的MIC相同。通过扩散技术，MIC与抑菌圈直径之间相关性良好；淋球菌的接种密度为2×10⁶CFU/ml。对青霉素、氨苄青霉素、甲砜霉素、四环素和壮观霉素采用了稀释和扩散技术。看来采用ABNG培养基的扩散技术会简化淋病奈瑟菌的抗生素敏感性检测，而稀释技术仍是参考方法。
