Verbaeys A, Ringoir S, Van Maele G, Lameire N
Department of Urology, University Hospital Gent, Belgium.
Urol Res. 1995;22(6):377-82. doi: 10.1007/BF00296879.
With sophisticated experiments it is necessary to handle laboratory animals many times. To determine the effect of minor handling a series of experiments was performed to measure the impact of fasting, anaesthesia, blood collection method and serum creatinine analysis on renal function. Simple clinical methods to measure renal function parameters such as diuresis, urinary osmolality, urinary creatinine excretion and serum creatinine were used. During fasting a significant increase (P < 0.01) in diuresis and a significant decrease (P < 0.01) in urinary osmolality were noted. Fasting and anaesthesia have the additional effect of significantly decreasing (P < 0.05) urinary creatinine excretion. Blood sampling method also has a significant impact on serum creatinine: venous sampling causes false-positive differences compared with simultaneous arterial sampling.
对于复杂的实验，有必要多次处理实验动物。为了确定轻微处理的影响，进行了一系列实验，以测量禁食、麻醉、采血方法和血清肌酐分析对肾功能的影响。使用了简单的临床方法来测量肾功能参数，如利尿、尿渗透压、尿肌酐排泄和血清肌酐。在禁食期间，观察到利尿显著增加（P < 0.01），尿渗透压显著降低（P < 0.01）。禁食和麻醉还具有显著降低（P < 0.05）尿肌酐排泄的额外作用。采血方法对血清肌酐也有显著影响：与同时进行的动脉采血相比，静脉采血会导致假阳性差异。
