Spillert C R, Ghuman S S, McGovern P J, Lazaro E J
Adv Shock Res. 1981;5:163-8.
Previous studies in our laboratory have demonstrated the protective effect of the spleen in murine burns. Since the spleen is a reservoir of the reticuloendothelial system (RES) and since small doses of endotoxin stimulate RES activity, the purpose of this investigation was to perform studies to determine whether endotoxin administration will further augment burn protection in splenectomized and nonsplenectomized animals. Swiss white mice were randomly assigned to one of two groups. One group was splenectomized and the other received a sham splenectomy. Twelve weeks later, under pentobarbital anesthesia, all animals were burned uniformly on the lower back. Log doses of Escherichia coli endotoxin or an equal volume of saline were administered intraperitoneally immediately after thermal injury. Severity of burns at 24 hours was assessed from gross and microscopic appearances using a grading scale of 0 (normal) to 4 (severe). Splenectomized controls receiving saline had more severe burns than the sham-splenectomized controls. Endotoxin decreased burn severity in both splenectomized and non-splenectomized mice. However, endotoxin afforded greater burn protection in the splenectomized mice when compared with the non-splenectomized animals.
我们实验室之前的研究已经证明了脾脏在小鼠烧伤中的保护作用。由于脾脏是网状内皮系统（RES）的一个储存库，且小剂量内毒素可刺激RES活性，本研究的目的是进行相关研究，以确定给予内毒素是否会进一步增强脾切除和未脾切除动物的烧伤保护作用。瑞士小白鼠被随机分为两组。一组进行脾切除，另一组接受假脾切除术。十二周后，在戊巴比妥麻醉下，所有动物的下背部均被均匀烧伤。热损伤后立即腹腔注射对数剂量的大肠杆菌内毒素或等体积的生理盐水。24小时时，根据大体和显微镜外观，使用0（正常）至4（严重）的分级量表评估烧伤严重程度。接受生理盐水的脾切除对照组比假脾切除对照组的烧伤更严重。内毒素降低了脾切除和未脾切除小鼠的烧伤严重程度。然而，与未脾切除的动物相比，内毒素对脾切除小鼠的烧伤保护作用更大。
