有机化学发光：氧化自由基反应的非侵入性检测方法。
Organ chemiluminescence: noninvasive assay for oxidative radical reactions.
作者信息
Boveris A, Cadenas E, Reiter R, Filipkowski M, Nakase Y, Chance B
出版信息
Proc Natl Acad Sci U S A. 1980 Jan;77(1):347-51. doi: 10.1073/pnas.77.1.347.
Abstract
In situ and perfused rat livers showed a spontaneous chemiluminescence of 7-12 counts/sec . cm2 (corresponding to 7-12 x 10(3) photons/sec . cm2); chemiluminescence was increased up to 30 times by infusion of exogenous hydroperoxides. The chemiluminescence of the perfused liver was oxygen dependent. Ethyl, t-butyl, and cumene hydroperoxides were almost equally effective in inducing light emission in the perfused liver. Glutathione release and chemiluminescence showed a parallel increase upon hydroperoxide supply to the perfused liver. A partial spectral analysis of the chemiluminescence of the perfused liver showed a predominance of red-light-emitting species, presumably arising from the singlet oxygen dimol-emission peaks. Many side reactions derived from the complex free radical sequence of lipid peroxidation could afford the chemistry leading to light emission, which represents only about 10(-14) of the utilization of peroxide.
摘要
原位及灌注大鼠肝脏显示出自发化学发光为7 - 12计数/秒·平方厘米（相当于7 - 12×10³光子/秒·平方厘米）；通过输注外源性氢过氧化物，化学发光增加至30倍。灌注肝脏的化学发光依赖于氧气。乙基、叔丁基和异丙苯氢过氧化物在诱导灌注肝脏发光方面几乎同样有效。向灌注肝脏供应氢过氧化物时，谷胱甘肽释放和化学发光呈平行增加。对灌注肝脏化学发光的部分光谱分析显示，主要是发出红光的物质，可能源于单线态氧双分子发射峰。脂质过氧化复杂自由基序列衍生的许多副反应可能提供导致发光的化学反应，而发光仅占过氧化物利用量的约10⁻¹⁴。
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