使用微溶性催化剂碘苷进行细胞表面放射性碘化。啮齿动物胸腺细胞分裂群体与非分裂群体之间的差异。
Cell-surface radioiodination with the sparingly soluble catalyst Iodogen. Difference between the dividing and non-dividing populations of rodent thymocytes.
作者信息
Salisbury J G, Graham J M
出版信息
Biochem J. 1981 Jan 15;194(1):351-5. doi: 10.1042/bj1940351.
Abstract
The surface proteins of dividing and non-dividing subpopulations of rat and mouse thymocytes have been labelled by using a new method of radioiodination. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and autoradiography of the labelled proteins shows distinct differences in labelling between the mouse and rat cells and also, in the case of the rat, between the dividing and non-dividing populations.
摘要
通过一种新的放射性碘化方法，已对大鼠和小鼠胸腺细胞的分裂亚群和非分裂亚群的表面蛋白进行了标记。对标记蛋白进行十二烷基硫酸钠/聚丙烯酰胺凝胶电泳和放射自显影分析，结果显示小鼠细胞和大鼠细胞之间在标记情况上存在明显差异，而且就大鼠而言，分裂群体和非分裂群体之间也存在差异。
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