孕早期和足月人胎盘碱性磷酸酶的体外合成与分泌
Synthesis and secretion of alkaline phosphatase in vitro from first-trimester and term human placentas.
作者信息
Galski H, Fridovich S E, Weinstein D, De Groot N, Segal S, Folman R, Hochberg A A
出版信息
Biochem J. 1981 Mar 15;194(3):857-66. doi: 10.1042/bj1940857.
The synthesis and secretion of alkaline phosphatases in vitro by human placental tissue incubated in organ culture were studied. First-trimester placenta synthesizes and secretes two different alkaline phosphatase isoenzymes (heat-labile and heat-stable), whereas in term placenta nearly all the alkaline phosphatase synthesized and secreted is heat-stable. The specific activities of alkaline phosphatases in first-trimester and term placental tissue remain constant throughout the time course of incubation. In the media, specific activities increase with time. Hence, alkaline phosphatase synthesis seems to be the driving force for its own secretion. The rates of synthesis de novo and of alkaline phosphatases were measured. The specific radioactivities of the secreted alkaline phosphatases were higher than the corresponding specific radioactivities in the tissue throughout the entire incubation period. The intracellular distribution of the alkaline phosphatase isoenzymes was compared.
研究了在器官培养中孵育的人胎盘组织体外碱性磷酸酶的合成与分泌。孕早期胎盘合成并分泌两种不同的碱性磷酸酶同工酶（热不稳定型和热稳定型），而足月胎盘合成和分泌的几乎所有碱性磷酸酶都是热稳定型的。孕早期和足月胎盘组织中碱性磷酸酶的比活性在整个孵育过程中保持恒定。在培养基中，比活性随时间增加。因此，碱性磷酸酶的合成似乎是其自身分泌的驱动力。测定了碱性磷酸酶的从头合成速率。在整个孵育期内，分泌的碱性磷酸酶的比放射性高于组织中相应的比放射性。比较了碱性磷酸酶同工酶的细胞内分布。
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