Lorberboum H, Weinstein D, Galski H, de Groot N, Segal S, Ilan J, Folman R, Hochberg A A
Mol Biol Rep. 1982 Mar 31;8(2):103-10. doi: 10.1007/BF00778512.
The in vitro synthesis of RNA in the human placental tissue, incubated in organ culture, was investigated. We followed the synthesis of the poly A(-) and poly A(+) RNA fractions, and investigated the distribution of the newly synthesized RNA among the subcellular fractions isolated from first and third trimester placentas. The poly A(-) RNA was the major fraction of the RNA synthesized in vitro. The incorporation of [3H]uridine into the poly A(+) RNA fraction was very low. As protein synthesis occurred during the entire incubation period, we suggest the presence of a pool of mRNA molecules in the form of mRNP particles.
我们研究了在器官培养中孵育的人胎盘组织中RNA的体外合成。我们追踪了多聚A（-）和多聚A（+）RNA组分的合成，并研究了新合成的RNA在从孕早期和孕晚期胎盘分离的亚细胞组分中的分布。多聚A（-）RNA是体外合成的RNA的主要组分。[3H]尿苷掺入多聚A（+）RNA组分的量非常低。由于在整个孵育期都发生了蛋白质合成，我们认为存在以mRNP颗粒形式存在的mRNA分子池。
