孕早期人蜕膜碱性磷酸酶的体外合成与分泌

The in vitro synthesis and secretion of alkaline phosphatase from first trimester human decidua.

作者信息

Galski H, Weinstein D, Abraham K, de Groot N, Segal S, Folman R, Hochberg A A

出版信息

Eur J Obstet Gynecol Reprod Biol. 1982 Oct;14(1):1-11. doi: 10.1016/0028-2243(82)90079-x.

Abstract

We studied the in vitro synthesis and secretion of alkaline phosphatase by human first trimester decidual tissue incubated in organ culture. Decidua synthesizes two different alkaline phosphatase isoenzymes; heat stable and heat labile. decidual tissue maintains a constant pool of alkaline phosphatases. The synthesis of alkaline phosphatase may be the driving force for its secretion. The de novo synthesis and secretion rate of alkaline phosphatases were also investigated. The higher specific radioactivities of the secreted enzymes than those found in the tissue may suggest that newly synthesized alkaline phosphatase is preferentially released. The intracellular distribution of the alkaline phosphatase isoenzymes was compared as well. The characteristics of the two isoenzymes are different in human decidua of maternal origin from those previously reported in the human placenta originating from embryonic stem cells.

摘要

我们研究了在器官培养中孵育的人孕早期蜕膜组织碱性磷酸酶的体外合成与分泌。蜕膜合成两种不同的碱性磷酸酶同工酶；热稳定型和热不稳定型。蜕膜组织维持着碱性磷酸酶的恒定储备。碱性磷酸酶的合成可能是其分泌的驱动力。还研究了碱性磷酸酶的从头合成与分泌速率。分泌酶的比放射性高于组织中的比放射性，这可能表明新合成的碱性磷酸酶优先释放。同时比较了碱性磷酸酶同工酶的细胞内分布。母体来源的人蜕膜中这两种同工酶的特性与先前报道的源自胚胎干细胞的人胎盘中的特性不同。