Isolation and sequence analysis of the intramembranous hydrophobic segment of the H-2Kb murine histocompatibility antigen.

The primary structure of the intramembranous segment (TC-1) of the mouse transplantation antigen, H-2Kb, has been determined. The segment contains a stretch of 31 uncharged amino acid residues and is localized between the NH2-terminal and the COOH-terminal hydrophilic regions of the molecule. The amino acid sequence of TC-1 is Trp-Asp-Glu-Pro-Pro-Ser-Thr-Val-Ser-Asn-Met-Ala-Thr-Val-Ala-Val-Leu-Val-Val-Leu -Gly-Ala-Ala-Ile-Val-Thr-Gly-Ala-Val-Val-Ala-Phe-Val-Met-Lys-Met-Arg-(Arg)-(Arg ).