Jarvie D R, Fell A F, Stewart M J
Clin Chim Acta. 1981 Dec 9;117(2):153-65. doi: 10.1016/0009-8981(81)90036-x.
A rapid method for the analysis of paraquat in plasma of poisoned patients is described. The dithionite colour reaction is used to detect paraquat, and matrix interference is eliminated by the use of a chemical deproteinization technique followed by second derivative spectroscopy of the reaction mixture. The method was compared with an established procedure using ion-pair extraction followed by dithionite reduction. Sensitivity and precision were comparable and the correlation in patients' plasma was good. The proposed direct method is rapid and suitable for emergency use in the management of patients poisoned with paraquat.
本文描述了一种用于分析中毒患者血浆中百草枯的快速方法。利用连二亚硫酸盐显色反应检测百草枯，并通过化学去蛋白技术消除基质干扰，随后对反应混合物进行二阶导数光谱分析。将该方法与一种既定的程序进行了比较，该程序采用离子对萃取，然后进行连二亚硫酸盐还原。灵敏度和精密度相当，患者血浆中的相关性良好。所提出的直接法快速，适用于百草枯中毒患者治疗中的紧急使用。
