New assay method for fatty acid synthetase with mass fragmentography. - Suppr | 超能文献

New assay method for fatty acid synthetase with mass fragmentography.

作者信息

Seyama Y, Kawaguchi A, Okuda S, Yamakawa T

出版信息

J Biochem. 1978 Nov;84(5):1309-14. doi: 10.1093/oxfordjournals.jbchem.a132250.

DOI:10.1093/oxfordjournals.jbchem.a132250

Abstract

A new assay method for fatty acid synthetase using mass fragmentography is described. The amounts of fatty acids synthesized in deuterated water are determined by mass fragmentography by monitoring the intensities of m/e 77 and m/e 74 fragment ions using heptadecanoic acid as an internal standard. This method is suitable for any fatty acid synthetase which produces several species of fatty acids and is also applicable to crude enzyme preparations. This procedure is more sensitive and specific than the conventional spectrophotometric method. Fatty acid synthetases from rat liver and Brevibacterium ammoniagenes are analyzed by this method.

摘要

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New assay method for fatty acid synthetase with mass fragmentography.

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Stereochemical studies of hydrogen incorporation from nucleotides with fatty acid synthetase from Brevibacterium ammoniagenes.产氨短杆菌脂肪酸合成酶催化核苷酸中氢掺入的立体化学研究。

J Biochem. 1977 Apr;81(4):1167-73. doi: 10.1093/oxfordjournals.jbchem.a131543.

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Stereochemical studies of hydrogen incorporation from nucleotides with fatty acid synthetase from Brevibacterium ammoniagenes.产氨短杆菌脂肪酸合成酶催化核苷酸中氢掺入的立体化学研究。

Adv Exp Med Biol. 1978;101:37-43. doi: 10.1007/978-1-4615-9071-2_4.

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Fatty acid synthetase from Brevibacterium ammoniagenes: formation of monounsaturated fatty acids by a multienzyme complex.产氨短杆菌的脂肪酸合成酶：多酶复合体催化单不饱和脂肪酸的形成。

Proc Natl Acad Sci U S A. 1977 Aug;74(8):3180-3. doi: 10.1073/pnas.74.8.3180.

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Differential hydrogen exchange during the fatty acid synthetase reaction: deuterium distribution of fatty acids synthesized from [2-2H2]malonyl-CoA.脂肪酸合成酶反应过程中的差异氢交换：由[2-²H₂]丙二酰辅酶A合成的脂肪酸的氘分布

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Incorporation of hydrogen atoms from deuterated water and stereospecifically deuterium-labeled nicotin amide nucleotides into fatty acids with the Escherichia coli fatty acid synthetase system.利用大肠杆菌脂肪酸合成酶系统将氘代水中的氢原子和立体特异性氘标记的烟酰胺核苷酸掺入脂肪酸中。

Biochim Biophys Acta. 1980 May 28;618(2):202-13.

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